DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients

Nadia Kontogianni; Niki Messini-Nikolaki; Konstantinos Christou; Konstantinos Gourgoulianis; Smaragdi Tsilimigaki; Stylianos M. Piperakis

doi:10.1002/em.20351

DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients

Nadia Kontogianni, Niki Messini-Nikolaki, Konstantinos Christou, Konstantinos Gourgoulianis, Smaragdi Tsilimigaki, Stylianos M. Piperakis

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen peroxide, ethanol, and γ-irradiation in lymphocytes from 35 OSA patients and 35 controls. We also measured the apoptosis and necrosis produced by these agents and the ability of the lymphocytes to repair the induced DNA damage. It was found that lymphocytes isolated from OSA patients had higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than lymphocytes from controls. OSA patients also had a reduced capacity to repair the DNA damage induced by the three agents, but apoptosis and necrosis were similar in OSA patients and the controls.

Original language	English
Pages (from-to)	722-727
Number of pages	6
Journal	Environmental and Molecular Mutagenesis
Volume	48
Issue number	9
DOIs	https://doi.org/10.1002/em.20351
Publication status	Published - 1 Dec 2007
Externally published	Yes

Keywords

Apoptosis
Comet assay
DNA damage
DNA repair
Obstructive sleep apnea syndrome

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10.1002/em.20351

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title = "DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients",

abstract = "Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen peroxide, ethanol, and γ-irradiation in lymphocytes from 35 OSA patients and 35 controls. We also measured the apoptosis and necrosis produced by these agents and the ability of the lymphocytes to repair the induced DNA damage. It was found that lymphocytes isolated from OSA patients had higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than lymphocytes from controls. OSA patients also had a reduced capacity to repair the DNA damage induced by the three agents, but apoptosis and necrosis were similar in OSA patients and the controls.",

keywords = "Apoptosis, Comet assay, DNA damage, DNA repair, Obstructive sleep apnea syndrome",

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T1 - DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients

AU - Kontogianni, Nadia

AU - Messini-Nikolaki, Niki

AU - Christou, Konstantinos

AU - Gourgoulianis, Konstantinos

AU - Tsilimigaki, Smaragdi

AU - Piperakis, Stylianos M.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen peroxide, ethanol, and γ-irradiation in lymphocytes from 35 OSA patients and 35 controls. We also measured the apoptosis and necrosis produced by these agents and the ability of the lymphocytes to repair the induced DNA damage. It was found that lymphocytes isolated from OSA patients had higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than lymphocytes from controls. OSA patients also had a reduced capacity to repair the DNA damage induced by the three agents, but apoptosis and necrosis were similar in OSA patients and the controls.

AB - Obstructive sleep apnea (OSA) syndrome is a respiratory disease that is linked to heart attacks and high blood pressure. In the present study, we used the Comet assay to compare basal DNA damage and DNA damage induction by hydrogen peroxide, ethanol, and γ-irradiation in lymphocytes from 35 OSA patients and 35 controls. We also measured the apoptosis and necrosis produced by these agents and the ability of the lymphocytes to repair the induced DNA damage. It was found that lymphocytes isolated from OSA patients had higher basal levels of DNA damage and were more sensitive to the effects of the DNA-damaging agents than lymphocytes from controls. OSA patients also had a reduced capacity to repair the DNA damage induced by the three agents, but apoptosis and necrosis were similar in OSA patients and the controls.

KW - Apoptosis

KW - Comet assay

KW - DNA damage

KW - DNA repair

KW - Obstructive sleep apnea syndrome

U2 - 10.1002/em.20351

DO - 10.1002/em.20351

M3 - Article

SN - 0893-6692

VL - 48

SP - 722

EP - 727

JO - Environmental and Molecular Mutagenesis

JF - Environmental and Molecular Mutagenesis

IS - 9

ER -

DNA damage and repair capacity in lymphocytes from obstructive sleep apnea patients

Abstract

Keywords

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