TY - JOUR
T1 - Diagnostic Performance of the Fujifilm SILVAMP TB-LAM in Children with Presumptive Tuberculosis
AU - Comella-Del-barrio, Patricia
AU - Molina-Moya, Bárbara
AU - Gautier, Jacqueline
AU - Villar-Hernández, Raquel
AU - Coute, Mariette Doresca Jean
AU - Sallés-Mingels, Beatriz
AU - Canales-Aliaga, Lydia
AU - Narcisse, Margarette
AU - Pérez-Porcuna, Tomás M.
AU - Creswell, Jacob
AU - Cuevas, Luis
AU - Domínguez, José
PY - 2021/4/28
Y1 - 2021/4/28
N2 - Current diagnostics for tuberculosis (TB) only manage to confirm a small proportion of children with TB and require respiratory samples, which are difficult to obtain. There is a need for non-invasive biomarker-based tests as an alternative to sputum testing. Fujifilm SILVAMP TB lipoarabinomannan (FujiLAM), a lateral-flow test to detect lipoarabinomannan in urine, is a novel non-sputum-based point-of-care diagnostic reported to have increased sensitivity for the diagnosis of TB among human immunodeficiency virus (HIV)-infected adults. We evaluate the performance of FujiLAM in children with presumptive TB. Fifty-nine children attending a paediatric hospital in Haiti with compatible signs and symptoms of TB were examined using Xpert MTB/RIF, smear microscopy and X-rays, and classified according to the certainty of diagnosis into bacteriologically confirmed TB ( = 5), unconfirmed TB (bacteriologically negative, = 50) and unlikely TB ( = 4). Healthy children ( = 20) were enrolled as controls. FujiLAM sensitivity and specificity were 60% and 95% among children with confirmed TB. FujiLAM's high specificity and its characteristics as a point-of-care indicate the test has a good potential for the diagnosis of TB in children.
AB - Current diagnostics for tuberculosis (TB) only manage to confirm a small proportion of children with TB and require respiratory samples, which are difficult to obtain. There is a need for non-invasive biomarker-based tests as an alternative to sputum testing. Fujifilm SILVAMP TB lipoarabinomannan (FujiLAM), a lateral-flow test to detect lipoarabinomannan in urine, is a novel non-sputum-based point-of-care diagnostic reported to have increased sensitivity for the diagnosis of TB among human immunodeficiency virus (HIV)-infected adults. We evaluate the performance of FujiLAM in children with presumptive TB. Fifty-nine children attending a paediatric hospital in Haiti with compatible signs and symptoms of TB were examined using Xpert MTB/RIF, smear microscopy and X-rays, and classified according to the certainty of diagnosis into bacteriologically confirmed TB ( = 5), unconfirmed TB (bacteriologically negative, = 50) and unlikely TB ( = 4). Healthy children ( = 20) were enrolled as controls. FujiLAM sensitivity and specificity were 60% and 95% among children with confirmed TB. FujiLAM's high specificity and its characteristics as a point-of-care indicate the test has a good potential for the diagnosis of TB in children.
KW - Children
KW - Diagnosis
KW - LAM
KW - Lipoarabinomannan
KW - Point-of-care
KW - Tuberculosis
KW - Urine
U2 - 10.3390/jcm10091914
DO - 10.3390/jcm10091914
M3 - Article
SN - 2077-0383
VL - 10
SP - 1914
JO - Journal of Clinical Medicine
JF - Journal of Clinical Medicine
IS - 9
M1 - 1914
ER -
