Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test

M. R. Salimi-Bejestani; J.W. McGarry; S. Felstead; P. Ortiz; A. Akca; Diana J.L. Williams

doi:10.1016/j.rvsc.2004.08.005

Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test

M. R. Salimi-Bejestani, J.W. McGarry, S. Felstead, P. Ortiz, A. Akca, Diana J.L. Williams

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

121 Citations (Scopus)

Abstract

An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	177-181
Number of pages	5
Journal	Research in Veterinary Science
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2004.08.005
Publication status	Published - 1 Apr 2005

Keywords

Cattle
ELISA
Fasciola hepatica
Immunodiagnosis

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10.1016/j.rvsc.2004.08.005

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title = "Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test",

abstract = "An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98\% (95\% confidence intervals, 96-100\%) and 96\% (95\% confidence intervals, 93-98\%) respectively at a cut-off value of 15\% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.",

keywords = "Cattle, ELISA, Fasciola hepatica, Immunodiagnosis",

author = "Salimi-Bejestani, \{M. R.\} and J.W. McGarry and S. Felstead and P. Ortiz and A. Akca and Williams, \{Diana J.L.\}",

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T1 - Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test

AU - Salimi-Bejestani, M. R.

AU - McGarry, J.W.

AU - Felstead, S.

AU - Ortiz, P.

AU - Akca, A.

AU - Williams, Diana J.L.

PY - 2005/4/1

Y1 - 2005/4/1

N2 - An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.

AB - An ELISA was developed for the detection of Fasciola hepatica antibody in serum of cattle. The assay was applied to sera from 258 naturally infected cattle, 256 non-infected cattle and six calves experimentally infected with F hepatica. The diagnostic sensitivity and specificity of the ELISA test was 98% (95% confidence intervals, 96-100%) and 96% (95% confidence intervals, 93-98%) respectively at a cut-off value of 15% positivity. The results using sera from the experimentally infected calves showed that antibodies were first detected 2-4 weeks after infection. The ELISA test was also compared to the commercially available Bio-X bovine F. hepatica ELISA kit. A subset of 39 positive sera and 47 negative sera were selected from the samples used to evaluate the in-house test. The results indicated that the agreement between the two tests was almost perfect (k statistic = 0.82). (C) 2004 Elsevier Ltd. All rights reserved.

KW - Cattle

KW - ELISA

KW - Fasciola hepatica

KW - Immunodiagnosis

U2 - 10.1016/j.rvsc.2004.08.005

DO - 10.1016/j.rvsc.2004.08.005

M3 - Article

SN - 0034-5288

VL - 78

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EP - 181

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -

Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test

Abstract

Keywords

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