Developing Wolbachia-Infected Cell Lines from Insects.

Mukund Madhav; Peter James

doi:10.1007/978-1-0716-3553-7_8

Developing Wolbachia-Infected Cell Lines from Insects.

Mukund Madhav, Peter James

Vector Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Wolbachia, an intracellular bacterium of arthropods, is an ideal candidate for use in the biological control of insect pests. The inability of Wolbachia to grow in the extracellular environment requires maintenance in live insects or insect cell cultures. Growing and adapting high-density Wolbachia in the targeted host cells improves the possibility of cross-species transinfection. Here, we describe a method for the establishment of a primary cell culture from insect embryos and its transinfection with Wolbachia.

Original language	English
Title of host publication	Methods in molecular biology (Clifton, N.J.)
Publisher	Springer Link
Pages	137-145
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-3553-7_8
Publication status	E-pub ahead of print - 26 Nov 2023

Keywords

Agricultural pest
Biocontrol
Ectoparasite
Haematobia spp
HIE-18 cell line

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10.1007/978-1-0716-3553-7_8

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KW - Agricultural pest

KW - Biocontrol

KW - Ectoparasite

KW - Haematobia spp

KW - HIE-18 cell line

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BT - Methods in molecular biology (Clifton, N.J.)

PB - Springer Link

ER -

Developing Wolbachia-Infected Cell Lines from Insects.

Abstract

Keywords

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