Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

Tracy Munthali Lunde; Adam Roberts; Mohammed Al-Haroni

doi:10.1080/20002297.2018.1552060

Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

Tracy Munthali Lunde, Adam Roberts, Mohammed Al-Haroni

Tropical Disease Biology

University of Tromsø – The Arctic University of Norway

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Background: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.

Objectives and Design: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.

Results: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.

Conclusions: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.

Original language	English
Article number	1552060
Pages (from-to)	1552060
Journal	Journal of Oral Microbiology
Volume	11
Issue number	1
Early online date	6 Dec 2018
DOIs	https://doi.org/10.1080/20002297.2018.1552060
Publication status	E-pub ahead of print - 6 Dec 2018

Keywords

antibiotic resistance
droplet digital PCR
mobile genetic elements (MGEs)
Oral streptococci
Tn916-Tn1545 family

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10.1080/20002297.2018.1552060Licence: CC BY

J Oral Microb 1 1 1552060 2019Final published version, 1.71 MBLicence: CC BY

Cite this

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title = "Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR",

abstract = "Background: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.Objectives and Design: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.Results: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.Conclusions: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.",

keywords = "antibiotic resistance, droplet digital PCR, mobile genetic elements (MGEs), Oral streptococci, Tn916-Tn1545 family",

author = "Lunde, \{Tracy Munthali\} and Adam Roberts and Mohammed Al-Haroni",

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T1 - Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

AU - Lunde, Tracy Munthali

AU - Roberts, Adam

AU - Al-Haroni, Mohammed

PY - 2018/12/6

Y1 - 2018/12/6

N2 - Background: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.Objectives and Design: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.Results: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.Conclusions: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.

AB - Background: Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements.Objectives and Design: In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In addition, we investigated the influence of tetracycline on the CR of Tn916-Tn1545 elements.Results: The ddPCR assay designed in this study is a reliable way to rapidly determine CN and CR of Tn916-Tn1545 elements.Conclusions: Our data also suggest that Tn916-Tn1545 elements are generally stable without selective pressure in the clinical oral Streptococcus strains investigated in this study.

KW - antibiotic resistance

KW - droplet digital PCR

KW - mobile genetic elements (MGEs)

KW - Oral streptococci

KW - Tn916-Tn1545 family

U2 - 10.1080/20002297.2018.1552060

DO - 10.1080/20002297.2018.1552060

M3 - Article

SN - 2000-2297

VL - 11

SP - 1552060

JO - Journal of Oral Microbiology

JF - Journal of Oral Microbiology

IS - 1

M1 - 1552060

ER -

Determination of copy number and circularization ratio of Tn916-Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR

Abstract

Keywords

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