TY - JOUR
T1 - Detection of high levels of anti-α-galactosyl antibodies in sera of patients with Old World cutaneous leishmaniasis: a possible tool for diagnosis and biomarker for cure in an elimination setting
AU - Al-Salem, Waleed S.
AU - Ferreira, Daniela
AU - Dyer, Naomi
AU - Alyamani, Essam J.
AU - Balghonaim, Salah M.
AU - Al-Mehna, Ahmed Y.
AU - Al-Zubiany, Saleem
AU - Ibrahim, El Keir
AU - Al Shahrani, Ali M.
AU - Alkhuailed, Hamed
AU - Aldahan, Mohammed A.
AU - Al Jarallh, Abdulaziz M.
AU - Abdelhady, Samer S.
AU - Al-Zahrani, Mohammed H.
AU - Almeida, Igor C.
AU - Acosta-Serrano, Alvaro
PY - 2014/10/9
Y1 - 2014/10/9
N2 - SUMMARY: In the Kingdom of Saudi Arabia (KSA), Old World cutaneous leishmaniasis (CL) is mainly caused by Leishmania major and Leishmania tropica parasites. Diagnosis of CL is predominately made by clinicians, who at times fail to detect the disease and are unable to identify parasite species. Here, we report the development of a chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) to measure the levels of anti-α-galactosyl antibodies in human sera. Using this assay, we have found that individuals infected with either Leishmania spp. had significantly elevated levels (up to 9-fold higher) of anti-α-Gal IgG compared to healthy control individuals. The assay sensitivity was 96% for L. major (95% CI; 94–98%) and 91% for L. tropica (95% CI; 86–98%) infections and therefore equivalent to restriction fragment length polymorphism-polymerase chain reaction analysis of parasite ITS1 gene. In addition, the assay had higher sensitivity than microscopy analysis, which only detected 68 and 45% of the L. major and L. tropica infections, respectively. Interestingly, up to 2 years following confirmed CL cure individuals had 28-fold higher levels of anti-α-Gal IgG compared to healthy volunteers. Monitoring levels of anti-α-Gal antibodies can be exploited as both a diagnostic tool and as a biomarker of cure of Old World CL in disease elimination settings.
AB - SUMMARY: In the Kingdom of Saudi Arabia (KSA), Old World cutaneous leishmaniasis (CL) is mainly caused by Leishmania major and Leishmania tropica parasites. Diagnosis of CL is predominately made by clinicians, who at times fail to detect the disease and are unable to identify parasite species. Here, we report the development of a chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) to measure the levels of anti-α-galactosyl antibodies in human sera. Using this assay, we have found that individuals infected with either Leishmania spp. had significantly elevated levels (up to 9-fold higher) of anti-α-Gal IgG compared to healthy control individuals. The assay sensitivity was 96% for L. major (95% CI; 94–98%) and 91% for L. tropica (95% CI; 86–98%) infections and therefore equivalent to restriction fragment length polymorphism-polymerase chain reaction analysis of parasite ITS1 gene. In addition, the assay had higher sensitivity than microscopy analysis, which only detected 68 and 45% of the L. major and L. tropica infections, respectively. Interestingly, up to 2 years following confirmed CL cure individuals had 28-fold higher levels of anti-α-Gal IgG compared to healthy volunteers. Monitoring levels of anti-α-Gal antibodies can be exploited as both a diagnostic tool and as a biomarker of cure of Old World CL in disease elimination settings.
KW - Anti-α-galactosyl antibodies
KW - chemiluminescent ELISA
KW - CL diagnosis
KW - Old World cutaneous leishmaniasis
U2 - 10.1017/s0031182014001607
DO - 10.1017/s0031182014001607
M3 - Article
SN - 0031-1820
VL - 141
SP - 1898
EP - 1903
JO - Parasitology
JF - Parasitology
IS - 14
ER -
