Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life

J. Bunn; W. G. MacKay; J. E. Thomas; D. C. Reid; L. T. Weaver

doi:10.1046/j.1472-765x.2002.01122.x

Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life

J. Bunn, W. G. MacKay, J. E. Thomas, D. C. Reid, L. T. Weaver

International Public Health

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

Aims: To provide evidence of water quality as a risk factor for acquisition of Helicobacter pylori in early life, and to identify evidence for its presence within pots used to store drinking water.

Methods and Results: A prospective cohort study of 65 infants was conducted in the rural village of Keneba, The Gambia. Age of H. pylori colonization was determined and water pot biofilms were tested for H. pylori by sequencing of amplified DNA. Use of supplemental water was a strong risk factor for H. pylori colonization in infants (OR 4.71, 95% CI 1.17-22.5). DNA with 95% homology to the 16S rRNA gene of H. pylori was isolated from biofilms of water pots. Conclusions: Drinking water may be a reservoir for H. pylori in areas of the developing world where water quality is poor. Early introduction of water, particularly if stored in, or collected from contaminated sources, may be associated with an increased rate of H. pylori colonization.

Original language	English
Pages (from-to)	450-454
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	34
Issue number	6
DOIs	https://doi.org/10.1046/j.1472-765x.2002.01122.x
Publication status	Published - 1 Jun 2002

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Cite this

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title = "Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life",

abstract = "Aims: To provide evidence of water quality as a risk factor for acquisition of Helicobacter pylori in early life, and to identify evidence for its presence within pots used to store drinking water. Methods and Results: A prospective cohort study of 65 infants was conducted in the rural village of Keneba, The Gambia. Age of H. pylori colonization was determined and water pot biofilms were tested for H. pylori by sequencing of amplified DNA. Use of supplemental water was a strong risk factor for H. pylori colonization in infants (OR 4.71, 95\% CI 1.17-22.5). DNA with 95\% homology to the 16S rRNA gene of H. pylori was isolated from biofilms of water pots. Conclusions: Drinking water may be a reservoir for H. pylori in areas of the developing world where water quality is poor. Early introduction of water, particularly if stored in, or collected from contaminated sources, may be associated with an increased rate of H. pylori colonization.",

author = "J. Bunn and MacKay, \{W. G.\} and Thomas, \{J. E.\} and Reid, \{D. C.\} and Weaver, \{L. T.\}",

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doi = "10.1046/j.1472-765x.2002.01122.x",

language = "English",

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TY - JOUR

T1 - Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life

AU - Bunn, J.

AU - MacKay, W. G.

AU - Thomas, J. E.

AU - Reid, D. C.

AU - Weaver, L. T.

PY - 2002/6/1

Y1 - 2002/6/1

N2 - Aims: To provide evidence of water quality as a risk factor for acquisition of Helicobacter pylori in early life, and to identify evidence for its presence within pots used to store drinking water. Methods and Results: A prospective cohort study of 65 infants was conducted in the rural village of Keneba, The Gambia. Age of H. pylori colonization was determined and water pot biofilms were tested for H. pylori by sequencing of amplified DNA. Use of supplemental water was a strong risk factor for H. pylori colonization in infants (OR 4.71, 95% CI 1.17-22.5). DNA with 95% homology to the 16S rRNA gene of H. pylori was isolated from biofilms of water pots. Conclusions: Drinking water may be a reservoir for H. pylori in areas of the developing world where water quality is poor. Early introduction of water, particularly if stored in, or collected from contaminated sources, may be associated with an increased rate of H. pylori colonization.

AB - Aims: To provide evidence of water quality as a risk factor for acquisition of Helicobacter pylori in early life, and to identify evidence for its presence within pots used to store drinking water. Methods and Results: A prospective cohort study of 65 infants was conducted in the rural village of Keneba, The Gambia. Age of H. pylori colonization was determined and water pot biofilms were tested for H. pylori by sequencing of amplified DNA. Use of supplemental water was a strong risk factor for H. pylori colonization in infants (OR 4.71, 95% CI 1.17-22.5). DNA with 95% homology to the 16S rRNA gene of H. pylori was isolated from biofilms of water pots. Conclusions: Drinking water may be a reservoir for H. pylori in areas of the developing world where water quality is poor. Early introduction of water, particularly if stored in, or collected from contaminated sources, may be associated with an increased rate of H. pylori colonization.

U2 - 10.1046/j.1472-765x.2002.01122.x

DO - 10.1046/j.1472-765x.2002.01122.x

M3 - Article

SN - 0266-8254

VL - 34

SP - 450

EP - 454

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 6

ER -

Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life

Abstract

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