Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing

Abdel Jelil Njouendou; Manuel Ritter; Chi Anizette Kien; Mathias E. Esum; Winston Patrick Chounna Ndongmo; Fanny Fri Fombad; Narcisse Victor T. Gandjui; Flobert Njiokou; Peter Enyong; Kenneth Pfarr; Joseph Turner; Laura E. Layland; Achim Hoerauf; Samuel Wanji

doi:10.1016/j.dib.2019.104930

Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing

Abdel Jelil Njouendou, Manuel Ritter, Chi Anizette Kien, Mathias E. Esum, Winston Patrick Chounna Ndongmo, Fanny Fri Fombad, Narcisse Victor T. Gandjui, Flobert Njiokou, Peter Enyong, Kenneth Pfarr, Joseph Turner, Laura E. Layland, Achim Hoerauf, Samuel Wanji

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The data presented here were collected from in vitro culture of M. perstans microfilariae, while asssessing the ability of two basic culture media; Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640) supplemented with 10% fetal bovine serum (FBS) and a monkey kidney epithelial cell line (LLC-MK2) to support the survival of this parasite stage; and their sussceptibility to chemotherapeutic agents. Parasites motlity were scored daily on a 4 scales system, and the average motility computed, as well as the area under the motility curve. Mortility was determined as the percentges of imotiled worms.

Original language	English
Article number	104930
Pages (from-to)	104930
Journal	Data in Brief
Volume	28
Early online date	4 Dec 2019
DOIs	https://doi.org/10.1016/j.dib.2019.104930
Publication status	Published - 1 Feb 2020

Keywords

In vitro culture system
Mansonella perstans
Microfilariae motility

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10.1016/j.dib.2019.104930Licence: CC BY-NC-ND

Datainbrief EP 2019 293Accepted author manuscript, 55.7 KBLicence: CC BY

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Njouendou, A. J., Ritter, M., Kien, C. A., Esum, M. E., Ndongmo, W. P. C., Fombad, F. F., Gandjui, N. V. T., Njiokou, F., Enyong, P., Pfarr, K., Turner, J., Layland, L. E., Hoerauf, A., & Wanji, S. (2020). Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing. Data in Brief, 28, 104930. Article 104930. https://doi.org/10.1016/j.dib.2019.104930

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title = "Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing",

abstract = "The data presented here were collected from in vitro culture of M. perstans microfilariae, while asssessing the ability of two basic culture media; Dulbecco{\textquoteright}s Modified Eagle{\textquoteright}s Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640) supplemented with 10\% fetal bovine serum (FBS) and a monkey kidney epithelial cell line (LLC-MK2) to support the survival of this parasite stage; and their sussceptibility to chemotherapeutic agents. Parasites motlity were scored daily on a 4 scales system, and the average motility computed, as well as the area under the motility curve. Mortility was determined as the percentges of imotiled worms.",

keywords = "In vitro culture system, Mansonella perstans, Microfilariae motility",

author = "Njouendou, \{Abdel Jelil\} and Manuel Ritter and Kien, \{Chi Anizette\} and Esum, \{Mathias E.\} and Ndongmo, \{Winston Patrick Chounna\} and Fombad, \{Fanny Fri\} and Gandjui, \{Narcisse Victor T.\} and Flobert Njiokou and Peter Enyong and Kenneth Pfarr and Joseph Turner and Layland, \{Laura E.\} and Achim Hoerauf and Samuel Wanji",

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doi = "10.1016/j.dib.2019.104930",

language = "English",

volume = "28",

pages = "104930",

journal = "Data in Brief",

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T1 - Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing

AU - Njouendou, Abdel Jelil

AU - Ritter, Manuel

AU - Kien, Chi Anizette

AU - Esum, Mathias E.

AU - Ndongmo, Winston Patrick Chounna

AU - Fombad, Fanny Fri

AU - Gandjui, Narcisse Victor T.

AU - Njiokou, Flobert

AU - Enyong, Peter

AU - Pfarr, Kenneth

AU - Turner, Joseph

AU - Layland, Laura E.

AU - Hoerauf, Achim

AU - Wanji, Samuel

PY - 2020/2/1

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N2 - The data presented here were collected from in vitro culture of M. perstans microfilariae, while asssessing the ability of two basic culture media; Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640) supplemented with 10% fetal bovine serum (FBS) and a monkey kidney epithelial cell line (LLC-MK2) to support the survival of this parasite stage; and their sussceptibility to chemotherapeutic agents. Parasites motlity were scored daily on a 4 scales system, and the average motility computed, as well as the area under the motility curve. Mortility was determined as the percentges of imotiled worms.

AB - The data presented here were collected from in vitro culture of M. perstans microfilariae, while asssessing the ability of two basic culture media; Dulbecco’s Modified Eagle’s Medium (DMEM) and Roswell Park Memorial Institute (RPMI-1640) supplemented with 10% fetal bovine serum (FBS) and a monkey kidney epithelial cell line (LLC-MK2) to support the survival of this parasite stage; and their sussceptibility to chemotherapeutic agents. Parasites motlity were scored daily on a 4 scales system, and the average motility computed, as well as the area under the motility curve. Mortility was determined as the percentges of imotiled worms.

KW - In vitro culture system

KW - Mansonella perstans

KW - Microfilariae motility

U2 - 10.1016/j.dib.2019.104930

DO - 10.1016/j.dib.2019.104930

M3 - Article

SN - 2352-3409

VL - 28

SP - 104930

JO - Data in Brief

JF - Data in Brief

M1 - 104930

ER -

Dataset on in vitro maintenance of Mansonella perstans microfilariae and drug testing

Abstract

Keywords

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