Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Lovett Lawson; Nanmdi Emenyonu; Saddiq T. Abdurrahman; Juliana O. Lawson; Gertrude N. Uzoewulu; Olumide M. Sogaolu; Juliana N. Ebisike; Christopher Parry; Mohammed A. Yassin; Luis Cuevas

doi:10.1186/1756-0500-6-215

Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Lovett Lawson
, Nanmdi Emenyonu
, Saddiq T. Abdurrahman
, Juliana O. Lawson
, Gertrude N. Uzoewulu
, Olumide M. Sogaolu
, Juliana N. Ebisike
, Christopher Parry
, Mohammed A. Yassin
, Luis Cuevas

Clinical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Background

This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.

Methods

This was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.

Results

527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.

Conclusions

There was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

Original language	English
Article number	215
Pages (from-to)	: 215
Journal	BMC Research Notes
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/1756-0500-6-215
Publication status	Published - 1 May 2013

Keywords

Drug sensitivity
First-line anti-TB drugs
Liquid culture
Multi-drug resistant tuberculosis
Solid culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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1756-0500-6-215Final published version, 169 KBLicence: CC BY

Cite this

@article{0c1d52190c5e4281af6d4432f3f7f184,

title = "Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria",

abstract = "BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81\%) were positive with BACTEC-MGIT-960, 59 (11\%) negative, 36 (7\%) contaminated and 4 (1\%) had non-tuberculosis mycobacteria (NTM). 411 (78\%) LJ cultures were positive, 89 (17\%) negative, 22 (4\%) contaminated and 5 (1\%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.",

keywords = "Drug sensitivity, First-line anti-TB drugs, Liquid culture, Multi-drug resistant tuberculosis, Solid culture",

author = "Lovett Lawson and Nanmdi Emenyonu and Abdurrahman, \{Saddiq T.\} and Lawson, \{Juliana O.\} and Uzoewulu, \{Gertrude N.\} and Sogaolu, \{Olumide M.\} and Ebisike, \{Juliana N.\} and Christopher Parry and Yassin, \{Mohammed A.\} and Luis Cuevas",

year = "2013",

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doi = "10.1186/1756-0500-6-215",

language = "English",

volume = "6",

pages = ": 215",

journal = "BMC Research Notes",

issn = "1756-0500",

publisher = "BioMed Central Ltd",

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TY - JOUR

T1 - Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

AU - Lawson, Lovett

AU - Emenyonu, Nanmdi

AU - Abdurrahman, Saddiq T.

AU - Lawson, Juliana O.

AU - Uzoewulu, Gertrude N.

AU - Sogaolu, Olumide M.

AU - Ebisike, Juliana N.

AU - Parry, Christopher

AU - Yassin, Mohammed A.

AU - Cuevas, Luis

PY - 2013/5/1

Y1 - 2013/5/1

N2 - BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

AB - BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

KW - Drug sensitivity

KW - First-line anti-TB drugs

KW - Liquid culture

KW - Multi-drug resistant tuberculosis

KW - Solid culture

U2 - 10.1186/1756-0500-6-215

DO - 10.1186/1756-0500-6-215

M3 - Article

SN - 1756-0500

VL - 6

SP - : 215

JO - BMC Research Notes

JF - BMC Research Notes

IS - 1

M1 - 215

ER -

Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Abstract

Keywords

UN SDGs

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