Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Lovett Lawson; Nanmdi Emenyonu; Saddiq T. Abdurrahman; Juliana O. Lawson; Gertrude N. Uzoewulu; Olumide M. Sogaolu; Juliana N. Ebisike; Christopher Parry; Mohammed A. Yassin; Luis Cuevas

doi:10.1186/1756-0500-6-215

Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Lovett Lawson, Nanmdi Emenyonu, Saddiq T. Abdurrahman, Juliana O. Lawson, Gertrude N. Uzoewulu, Olumide M. Sogaolu, Juliana N. Ebisike, Christopher Parry, Mohammed A. Yassin, Luis Cuevas

Clinical Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Background

This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.

Methods

This was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.

Results

527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.

Conclusions

There was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

Original language	English
Article number	215
Pages (from-to)	: 215
Journal	BMC Research Notes
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/1756-0500-6-215
Publication status	Published - 1 May 2013

Keywords

Drug sensitivity
First-line anti-TB drugs
Liquid culture
Multi-drug resistant tuberculosis
Solid culture

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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1756-0500-6-215Final published version, 169 KBLicence: CC BY

Cite this

@article{0c1d52190c5e4281af6d4432f3f7f184,

title = "Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria",

abstract = "BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81\%) were positive with BACTEC-MGIT-960, 59 (11\%) negative, 36 (7\%) contaminated and 4 (1\%) had non-tuberculosis mycobacteria (NTM). 411 (78\%) LJ cultures were positive, 89 (17\%) negative, 22 (4\%) contaminated and 5 (1\%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.",

keywords = "Drug sensitivity, First-line anti-TB drugs, Liquid culture, Multi-drug resistant tuberculosis, Solid culture",

author = "Lovett Lawson and Nanmdi Emenyonu and Abdurrahman, \{Saddiq T.\} and Lawson, \{Juliana O.\} and Uzoewulu, \{Gertrude N.\} and Sogaolu, \{Olumide M.\} and Ebisike, \{Juliana N.\} and Christopher Parry and Yassin, \{Mohammed A.\} and Luis Cuevas",

year = "2013",

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doi = "10.1186/1756-0500-6-215",

language = "English",

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journal = "BMC Research Notes",

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TY - JOUR

T1 - Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

AU - Lawson, Lovett

AU - Emenyonu, Nanmdi

AU - Abdurrahman, Saddiq T.

AU - Lawson, Juliana O.

AU - Uzoewulu, Gertrude N.

AU - Sogaolu, Olumide M.

AU - Ebisike, Juliana N.

AU - Parry, Christopher

AU - Yassin, Mohammed A.

AU - Cuevas, Luis

PY - 2013/5/1

Y1 - 2013/5/1

N2 - BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

AB - BackgroundThis study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.MethodsThis was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol.Results527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases.ConclusionsThere was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics.

KW - Drug sensitivity

KW - First-line anti-TB drugs

KW - Liquid culture

KW - Multi-drug resistant tuberculosis

KW - Solid culture

U2 - 10.1186/1756-0500-6-215

DO - 10.1186/1756-0500-6-215

M3 - Article

SN - 1756-0500

VL - 6

SP - : 215

JO - BMC Research Notes

JF - BMC Research Notes

IS - 1

M1 - 215

ER -

Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

Abstract

Keywords

UN SDGs

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