Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

D. von Blumroder; G. Schares; R. Norton; Diana J.L. Williams; I. Esteban-Redondo; S. Wright; C. Bjorkman; J. Frossling; V. Risco-Castillo; A. Fernandez-Garcia; L. M. Ortega-Mora; H. Sager; A. Hemphill; C. van Maanen; W. Wouda; F. J. Conraths

doi:10.1016/j.vetpar.2003.12.010

Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

D. von Blumroder, G. Schares, R. Norton, Diana J.L. Williams, I. Esteban-Redondo, S. Wright, C. Bjorkman, J. Frossling, V. Risco-Castillo, A. Fernandez-Garcia, L. M. Ortega-Mora, H. Sager, A. Hemphill, C. van Maanen, W. Wouda, F. J. Conraths

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

84 Citations (Scopus)

Abstract

Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	11-22
Number of pages	12
Journal	Veterinary Parasitology
Volume	120
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetpar.2003.12.010
Publication status	Published - 26 Feb 2004

Keywords

Cut-off
Enzyme-linked immunosorbent assay
Indirect fluorescent antibody test
Neospora caninum
Test standardisation
TG-ROC

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10.1016/j.vetpar.2003.12.010

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von Blumroder, D., Schares, G., Norton, R., Williams, D. J. L., Esteban-Redondo, I., Wright, S., Bjorkman, C., Frossling, J., Risco-Castillo, V., Fernandez-Garcia, A., Ortega-Mora, L. M., Sager, H., Hemphill, A., van Maanen, C., Wouda, W., & Conraths, F. J. (2004). Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines. Veterinary Parasitology, 120(1-2), 11-22. https://doi.org/10.1016/j.vetpar.2003.12.010

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title = "Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines",

abstract = "Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.",

keywords = "Cut-off, Enzyme-linked immunosorbent assay, Indirect fluorescent antibody test, Neospora caninum, Test standardisation, TG-ROC",

author = "\{von Blumroder\}, D. and G. Schares and R. Norton and Williams, \{Diana J.L.\} and I. Esteban-Redondo and S. Wright and C. Bjorkman and J. Frossling and V. Risco-Castillo and A. Fernandez-Garcia and Ortega-Mora, \{L. M.\} and H. Sager and A. Hemphill and \{van Maanen\}, C. and W. Wouda and Conraths, \{F. J.\}",

year = "2004",

month = feb,

day = "26",

doi = "10.1016/j.vetpar.2003.12.010",

language = "English",

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von Blumroder, D, Schares, G, Norton, R, Williams, DJL, Esteban-Redondo, I, Wright, S, Bjorkman, C, Frossling, J, Risco-Castillo, V, Fernandez-Garcia, A, Ortega-Mora, LM, Sager, H, Hemphill, A, van Maanen, C, Wouda, W & Conraths, FJ 2004, 'Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines', Veterinary Parasitology, vol. 120, no. 1-2, pp. 11-22. https://doi.org/10.1016/j.vetpar.2003.12.010

TY - JOUR

T1 - Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

AU - von Blumroder, D.

AU - Schares, G.

AU - Norton, R.

AU - Williams, Diana J.L.

AU - Esteban-Redondo, I.

AU - Wright, S.

AU - Bjorkman, C.

AU - Frossling, J.

AU - Risco-Castillo, V.

AU - Fernandez-Garcia, A.

AU - Ortega-Mora, L. M.

AU - Sager, H.

AU - Hemphill, A.

AU - van Maanen, C.

AU - Wouda, W.

AU - Conraths, F. J.

PY - 2004/2/26

Y1 - 2004/2/26

N2 - Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

AB - Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

KW - Cut-off

KW - Enzyme-linked immunosorbent assay

KW - Indirect fluorescent antibody test

KW - Neospora caninum

KW - Test standardisation

KW - TG-ROC

U2 - 10.1016/j.vetpar.2003.12.010

DO - 10.1016/j.vetpar.2003.12.010

M3 - Article

SN - 0304-4017

VL - 120

SP - 11

EP - 22

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 1-2

ER -

Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

Abstract

Keywords

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