TY - JOUR
T1 - Comparative study of haemagglutination activity in the haemolymph of three tsetse fly Glossina species
AU - Ingram, George A.
AU - Molyneux, David
PY - 1993/11/1
Y1 - 1993/11/1
N2 - 1. 1. Glossina morsitans morsitans (Gmm), G. palpalis gambiensis (Gpg) and G. tachinoides (Gt) haemolymph possessed multiple, glycoproteinaceous haemagglutinins (HGN). 2. 2. Tsetse HGN bind to human erythrocyte surface glycoprotein/glycopeptide residues or, with Gmm and Gpg anti-0 activity, glycolipid moieties. 3. 3. Variations in HGN physico-chemical properties occurred between the morsitans (Gmm) and palpalis (Gpg and Gt), and amongst the palpalis, groups of flies with respect to relative heat-lability, susceptibility to dithiothreitol reduction, resistance to γ-radiation exposure and sensitivity to urea treatment. 4. 4. Gt and Gmm required acid and acid to neutral conditions respectively, and Ca2+ ion presence, for optimum agglutination activity whilst Gpg required neutral to alkaline pH and Mg2+ ions. 5. 5. The findings reported here provide further information regarding HGN (lectin) properties in different species of the genus Glossina, member of the Diptera, a little studied order with respect to insect vector immunity.
AB - 1. 1. Glossina morsitans morsitans (Gmm), G. palpalis gambiensis (Gpg) and G. tachinoides (Gt) haemolymph possessed multiple, glycoproteinaceous haemagglutinins (HGN). 2. 2. Tsetse HGN bind to human erythrocyte surface glycoprotein/glycopeptide residues or, with Gmm and Gpg anti-0 activity, glycolipid moieties. 3. 3. Variations in HGN physico-chemical properties occurred between the morsitans (Gmm) and palpalis (Gpg and Gt), and amongst the palpalis, groups of flies with respect to relative heat-lability, susceptibility to dithiothreitol reduction, resistance to γ-radiation exposure and sensitivity to urea treatment. 4. 4. Gt and Gmm required acid and acid to neutral conditions respectively, and Ca2+ ion presence, for optimum agglutination activity whilst Gpg required neutral to alkaline pH and Mg2+ ions. 5. 5. The findings reported here provide further information regarding HGN (lectin) properties in different species of the genus Glossina, member of the Diptera, a little studied order with respect to insect vector immunity.
U2 - 10.1016/0305-0491(93)90133-p
DO - 10.1016/0305-0491(93)90133-p
M3 - Article
SN - 1096-4959
VL - 106
SP - 563
EP - 573
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 3
ER -