Comparative performance evaluation of blood film microscopy for the
diagnosis of bovine trypanosomosis by some laboratories in North-central
Nigeria

Jones Soladoye Akinbobola; Solomon Oluwole Okaiyeto; Anthony Kojo Sackey; Lushaikyaa Allam; Bisalla Mohammed; Jerome Nyhalah Dinga; Prosper C. Chukwuemeka; Lucas Cunningham

doi:10.5455/ovj.2023.v13.i5.12

Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria

Jones Soladoye Akinbobola
, Solomon Oluwole Okaiyeto
, Anthony Kojo Sackey
, Lushaikyaa Allam
, Bisalla Mohammed
, Jerome Nyhalah Dinga
, Prosper C. Chukwuemeka
, Lucas Cunningham

University of Abuja, Gwagwalada
Ahmadu Bello University
Michael Gahnyam Gbeugvat Foundation
University of Buea

Research output: Contribution to journal › Article › peer-review

Abstract

Background:

Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.

Aim:

This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.

Methods:

Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.

Results:

All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.

Conclusion:

Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.

Original language	English
Pages (from-to)	599-603
Number of pages	5
Journal	Open Veterinary Journal
Volume	13
Issue number	5
DOIs	https://doi.org/10.5455/ovj.2023.v13.i5.12
Publication status	Published - 13 May 2023

Keywords

Blood film microscopy
Bovine trypanosomosis
Evaluation
North-central Nigeria
Performance

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10.5455/ovj.2023.v13.i5.12Licence: CC BY-NC

100-1665657894Final published version, 818 KBLicence: CC BY-NC

Cite this

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title = "Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria",

abstract = "Background: Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.Aim:This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.Methods: Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.Results:All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.Conclusion:Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.",

keywords = "Blood film microscopy, Bovine trypanosomosis, Evaluation, North-central Nigeria, Performance",

author = "Akinbobola, \{Jones Soladoye\} and Okaiyeto, \{Solomon Oluwole\} and Sackey, \{Anthony Kojo\} and Lushaikyaa Allam and Bisalla Mohammed and Dinga, \{Jerome Nyhalah\} and Chukwuemeka, \{Prosper C.\} and Lucas Cunningham",

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Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria. / Akinbobola, Jones Soladoye; Okaiyeto, Solomon Oluwole; Sackey, Anthony Kojo et al.
In: Open Veterinary Journal, Vol. 13, No. 5, 13.05.2023, p. 599-603.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria

AU - Akinbobola, Jones Soladoye

AU - Okaiyeto, Solomon Oluwole

AU - Sackey, Anthony Kojo

AU - Allam, Lushaikyaa

AU - Mohammed, Bisalla

AU - Dinga, Jerome Nyhalah

AU - Chukwuemeka, Prosper C.

AU - Cunningham, Lucas

PY - 2023/5/13

Y1 - 2023/5/13

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AB - Background: Due to its affordability in disease-affected communities and suitability for field application, microscopy has historically been considered the gold standard for field diagnosis of trypanosomosis in rural settings.Aim:This works aims to compare the performance of microscopists on bovine trypanosome microscopy by organizing the first comparative assessment on a correct reading of slides by laboratory professionals using the read slide results and a structured interviewer-administered questionnaire in North-central Nigeria.Methods: Ten participants were addressed, as they were sent a panel of two slides (Slide 1: No Trypanosome present; Slide 2: Trypanosome present) and a questionnaire.Results:All participants greater than 41 years old reported correctly the presence and absence of parasites on slides. Only 3/8 of microscopists from routine diagnostic laboratories reported correctly the presence of the parasite.Conclusion:Our study confirmed errors in reading slides. Therefore, training of microscopists besides a nationwide quality assessment is recommended.

KW - Blood film microscopy

KW - Bovine trypanosomosis

KW - Evaluation

KW - North-central Nigeria

KW - Performance

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JO - Open Veterinary Journal

JF - Open Veterinary Journal

IS - 5

ER -

Comparative performance evaluation of blood film microscopy for the diagnosis of bovine trypanosomosis by some laboratories in North-central Nigeria

Abstract

Keywords

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