Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt.

Joachim R. de Miranda; Laura Brettell; Nor Chejanovsky; Anna K. Childers; Anne Dalmon; Ward Deboutte; Dirk C. de Graaf; Vincent Doublet; Haftom Gebremedhn; Elke Genersch; Sebastian Gisder; Fredrik Granberg; Nizar J. Haddad; Rene Kaden; Robyn Manley; Jelle Matthijnssens; Ivan Meeus; Hussein Migdadi; Meghan O. Milbrath; Fanny Mondet; Emily J. Remnant; John M.K. Roberts; Eugene V. Ryabov; Noa Sela; Guy Smagghe; Hema Somanathan; Lena Wilfert; Owen N. Wright; Stephen J. Martin; Brenda V. Ball

doi:10.1186/s12985-022-01740-2

Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt.

Joachim R. de Miranda
, Laura Brettell
, Nor Chejanovsky
, Anna K. Childers
, Anne Dalmon
, Ward Deboutte
, Dirk C. de Graaf
, Vincent Doublet
, Haftom Gebremedhn
, Elke Genersch
, Sebastian Gisder
, Fredrik Granberg
, Nizar J. Haddad
, Rene Kaden
, Robyn Manley
, Jelle Matthijnssens
, Ivan Meeus
, Hussein Migdadi
, Meghan O. Milbrath
, Fanny Mondet

Emily J. Remnant, John M.K. Roberts, Eugene V. Ryabov, Noa Sela, Guy Smagghe, Hema Somanathan, Lena Wilfert, Owen N. Wright, Stephen J. Martin, Brenda V. Ball

Vector Biology

Swedish University of Agricultural Sciences
Western Sydney University
University of Salford
Agricultural Research Organization of Israel
United States Department of Agriculture
INRAE
KU Leuven
Max Planck Institute of Immunobiology and Epigenetics
Ghent University
University of Exeter
Ulm University
Tigray Health Research Institute
Free University of Berlin
Institute for Bee Research
National Agricultural Research Center
Uppsala University
University of Sydney
CSIRO
Indian Institute of Science Education and Research Thiruvananthapuram
Rothamsted Research

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

In 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.

Original language	English
Article number	12
Pages (from-to)	12
Journal	Virology Journal
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12985-022-01740-2
Publication status	Published - 15 Jan 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apis mellifera
Bioinformatic screening
Deformed wing virus
Egypt bee virus
Honeybee
Master strain
RNA sequencing
Varroa destructor
Western blot

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10.1186/s12985-022-01740-2Licence: CC BY

2022deMiranda - Cold case disappearance EBV fourth DWV master variant 1970s EgyptFinal published version, 2.44 MBLicence: CC BY

Cite this

de Miranda, J. R., Brettell, L., Chejanovsky, N., Childers, A. K., Dalmon, A., Deboutte, W., de Graaf, D. C., Doublet, V., Gebremedhn, H., Genersch, E., Gisder, S., Granberg, F., Haddad, N. J., Kaden, R., Manley, R., Matthijnssens, J., Meeus, I., Migdadi, H., Milbrath, M. O., ... Ball, B. V. (2022). Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt. Virology Journal, 19(1), 12. Article 12. https://doi.org/10.1186/s12985-022-01740-2

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title = "Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt.",

abstract = "In 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.",

keywords = "Apis mellifera, Bioinformatic screening, Deformed wing virus, Egypt bee virus, Honeybee, Master strain, RNA sequencing, Varroa destructor, Western blot",

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de Miranda, JR, Brettell, L, Chejanovsky, N, Childers, AK, Dalmon, A, Deboutte, W, de Graaf, DC, Doublet, V, Gebremedhn, H, Genersch, E, Gisder, S, Granberg, F, Haddad, NJ, Kaden, R, Manley, R, Matthijnssens, J, Meeus, I, Migdadi, H, Milbrath, MO, Mondet, F, Remnant, EJ, Roberts, JMK, Ryabov, EV, Sela, N, Smagghe, G, Somanathan, H, Wilfert, L, Wright, ON, Martin, SJ & Ball, BV 2022, 'Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt.', Virology Journal, vol. 19, no. 1, 12, pp. 12. https://doi.org/10.1186/s12985-022-01740-2

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AU - de Miranda, Joachim R.

AU - Brettell, Laura

AU - Chejanovsky, Nor

AU - Childers, Anna K.

AU - Dalmon, Anne

AU - Deboutte, Ward

AU - de Graaf, Dirk C.

AU - Doublet, Vincent

AU - Gebremedhn, Haftom

AU - Genersch, Elke

AU - Gisder, Sebastian

AU - Granberg, Fredrik

AU - Haddad, Nizar J.

AU - Kaden, Rene

AU - Manley, Robyn

AU - Matthijnssens, Jelle

AU - Meeus, Ivan

AU - Migdadi, Hussein

AU - Milbrath, Meghan O.

AU - Mondet, Fanny

AU - Remnant, Emily J.

AU - Roberts, John M.K.

AU - Ryabov, Eugene V.

AU - Sela, Noa

AU - Smagghe, Guy

AU - Somanathan, Hema

AU - Wilfert, Lena

AU - Wright, Owen N.

AU - Martin, Stephen J.

AU - Ball, Brenda V.

PY - 2022/1/15

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N2 - In 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.

AB - In 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.

KW - Apis mellifera

KW - Bioinformatic screening

KW - Deformed wing virus

KW - Egypt bee virus

KW - Honeybee

KW - Master strain

KW - RNA sequencing

KW - Varroa destructor

KW - Western blot

U2 - 10.1186/s12985-022-01740-2

DO - 10.1186/s12985-022-01740-2

M3 - Article

SN - 1743-422X

VL - 19

SP - 12

JO - Virology Journal

JF - Virology Journal

IS - 1

M1 - 12

ER -

Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt.

Abstract

UN SDGs

Keywords

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