Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

Paul Carroll; Julian Muwanguzi-Karugaba; Tanya Parish

doi:10.1186/s13104-018-3798-3

Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

Paul Carroll
, Julian Muwanguzi-Karugaba
, Tanya Parish

Queen Mary University of London
Infectious Disease Research Institute

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Objective: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. Results: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (P_hsp60, P_rpsA or P_G13) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria.

Original language	English
Article number	685
Journal	BMC Research Notes
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/s13104-018-3798-3
Publication status	Published - 1 Oct 2018
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Fluorescent protein
Mycobacteria
Reporter system

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10.1186/s13104-018-3798-3Licence: CC BY

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AB - Objective: We have previously codon-optimized a number of red fluorescent proteins for use in Mycobacterium tuberculosis (mCherry, tdTomato, Turbo-635). We aimed to expand this repertoire to include DsRed, another widely used and flexible red fluorescent protein. Results: We generated expression constructs with a full length DsRed under the control of one of three strong, constitutive promoters (Phsp60, PrpsA or PG13) for use in mycobacteria. We confirmed that full length DsRed (225 amino acids) was expressed and fluoresced brightly. In contrast to mCherry, truncated versions of DsRed lacking several amino acids at the N-terminus were not functional. Thus, we have expanded the repertoire of optimized fluorescent proteins for mycobacteria.

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KW - Reporter system

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Codon-optimized DsRed fluorescent protein for use in Mycobacterium tuberculosis

Abstract

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Keywords

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