Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family

Mark Paine; Ana M. Moura‐Da‐Silva; R. David G. Theakston; Julian M. Crampton

doi:10.1111/j.1432-1033.1994.00483.x

Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family

Mark Paine
, Ana M. Moura‐Da‐Silva
, R. David G. Theakston
, Julian M. Crampton

Vector Biology

Liverpool School of Tropical Medicine
Instituto Butantan

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

The genes coding for two metalloproteases, EcH‐I and EcH‐II, have been cloned from an Echis pyramidum leakeyi venom gland cDNA library. The cDNA sequences predict two zymogen molecules with strong amino acid sequence similarity and the same domain structure present in other members of the viper metalloprotease/disintegrin gene family. EcH‐I and EcH‐II contain pro‐protein, enzyme and disintegrin domains. Analysis of the cDNAs coding for EcH‐I, EcH‐II, jararhagin, trigramin and Ht‐e reveals a strong similarity, particularly in the untranslated regions and regions coding for the pro‐peptide. Comparison of EcH‐I and EcH‐II with venom metalloproteases, mammalian matrix‐degrading metalloproteases, sperm proteins, and a potential tumour suppressor gene highlights the presence of a number of motifs with potential functional significance.

Original language	English
Pages (from-to)	483-488
Number of pages	6
Journal	FEBS Journal
Volume	224
Issue number	2
DOIs	https://doi.org/10.1111/j.1432-1033.1994.00483.x
Publication status	Published - 1 Sept 1994

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Paine, M., Moura‐Da‐Silva, A. M., Theakston, R. D. G., & Crampton, J. M. (1994). Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family. FEBS Journal, 224(2), 483-488. https://doi.org/10.1111/j.1432-1033.1994.00483.x

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title = "Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family",

abstract = "The genes coding for two metalloproteases, EcH‐I and EcH‐II, have been cloned from an Echis pyramidum leakeyi venom gland cDNA library. The cDNA sequences predict two zymogen molecules with strong amino acid sequence similarity and the same domain structure present in other members of the viper metalloprotease/disintegrin gene family. EcH‐I and EcH‐II contain pro‐protein, enzyme and disintegrin domains. Analysis of the cDNAs coding for EcH‐I, EcH‐II, jararhagin, trigramin and Ht‐e reveals a strong similarity, particularly in the untranslated regions and regions coding for the pro‐peptide. Comparison of EcH‐I and EcH‐II with venom metalloproteases, mammalian matrix‐degrading metalloproteases, sperm proteins, and a potential tumour suppressor gene highlights the presence of a number of motifs with potential functional significance.",

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Paine, M, Moura‐Da‐Silva, AM, Theakston, RDG & Crampton, JM 1994, 'Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family', FEBS Journal, vol. 224, no. 2, pp. 483-488. https://doi.org/10.1111/j.1432-1033.1994.00483.x

Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family. / Paine, Mark; Moura‐Da‐Silva, Ana M.; Theakston, R. David G. et al.
In: FEBS Journal, Vol. 224, No. 2, 01.09.1994, p. 483-488.

Research output: Contribution to journal › Article › peer-review

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T1 - Cloning of Metalloprotease Genes in the Carpet Viper (Echis pyramidurm leakeyi): Further Members of the Metalloprotease/Disintegrin Gene Family: Further Members of the Metalloprotease/Disintegrin Gene Family

AU - Paine, Mark

AU - Moura‐Da‐Silva, Ana M.

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AU - Crampton, Julian M.

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AB - The genes coding for two metalloproteases, EcH‐I and EcH‐II, have been cloned from an Echis pyramidum leakeyi venom gland cDNA library. The cDNA sequences predict two zymogen molecules with strong amino acid sequence similarity and the same domain structure present in other members of the viper metalloprotease/disintegrin gene family. EcH‐I and EcH‐II contain pro‐protein, enzyme and disintegrin domains. Analysis of the cDNAs coding for EcH‐I, EcH‐II, jararhagin, trigramin and Ht‐e reveals a strong similarity, particularly in the untranslated regions and regions coding for the pro‐peptide. Comparison of EcH‐I and EcH‐II with venom metalloproteases, mammalian matrix‐degrading metalloproteases, sperm proteins, and a potential tumour suppressor gene highlights the presence of a number of motifs with potential functional significance.

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