Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata

Katherine A. Murray; Rory J. Post; Julian M. Crampton; Philip McCall; Boubacar Kouyate

doi:10.1016/0166-6851(88)90089-8

Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata

Katherine A. Murray, Rory J. Post, Julian M. Crampton, Philip McCall, Boubacar Kouyate

Vector Biology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Two clones, pOA1 and pOA5, have been isolated from a genomic DNA library prepared from pools of Onchocerca armillata adults in the plasmid vector pUC12. In dot-blot hybridisations, these two clones do not cross-hybridise significantly with total genomic DNA from O. volvulus, O. gutturosa, O. ochengi, O. gibsoni, O. lienalis, bovine, human, Culicoides nubeculosus, Simulium species or Brugia pahangi, but do hybridise with as little as 100 pg of DNA from two separate geographic isolates of O. armillata. The sequence of pOA1 and pOA5 has been determined and found to contain a repetitive DNA sequence 147 bp in length. These clones can be used as specific and sensitive DNA probes for the identification of O. armillata capable of identifying a single L3 larva.

Original language	English
Pages (from-to)	209-215
Number of pages	7
Journal	Molecular and Biochemical Parasitology
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/0166-6851(88)90089-8
Publication status	Published - 1 Sept 1988

Keywords

DNA probe
Onchocerca armillata
Repetitive DNA
Taxonomy

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10.1016/0166-6851(88)90089-8

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title = "Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata",

abstract = "Two clones, pOA1 and pOA5, have been isolated from a genomic DNA library prepared from pools of Onchocerca armillata adults in the plasmid vector pUC12. In dot-blot hybridisations, these two clones do not cross-hybridise significantly with total genomic DNA from O. volvulus, O. gutturosa, O. ochengi, O. gibsoni, O. lienalis, bovine, human, Culicoides nubeculosus, Simulium species or Brugia pahangi, but do hybridise with as little as 100 pg of DNA from two separate geographic isolates of O. armillata. The sequence of pOA1 and pOA5 has been determined and found to contain a repetitive DNA sequence 147 bp in length. These clones can be used as specific and sensitive DNA probes for the identification of O. armillata capable of identifying a single L3 larva.",

keywords = "DNA probe, Onchocerca armillata, Repetitive DNA, Taxonomy",

author = "Murray, \{Katherine A.\} and Post, \{Rory J.\} and Crampton, \{Julian M.\} and Philip McCall and Boubacar Kouyate",

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T1 - Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata

AU - Murray, Katherine A.

AU - Post, Rory J.

AU - Crampton, Julian M.

AU - McCall, Philip

AU - Kouyate, Boubacar

PY - 1988/9/1

Y1 - 1988/9/1

N2 - Two clones, pOA1 and pOA5, have been isolated from a genomic DNA library prepared from pools of Onchocerca armillata adults in the plasmid vector pUC12. In dot-blot hybridisations, these two clones do not cross-hybridise significantly with total genomic DNA from O. volvulus, O. gutturosa, O. ochengi, O. gibsoni, O. lienalis, bovine, human, Culicoides nubeculosus, Simulium species or Brugia pahangi, but do hybridise with as little as 100 pg of DNA from two separate geographic isolates of O. armillata. The sequence of pOA1 and pOA5 has been determined and found to contain a repetitive DNA sequence 147 bp in length. These clones can be used as specific and sensitive DNA probes for the identification of O. armillata capable of identifying a single L3 larva.

AB - Two clones, pOA1 and pOA5, have been isolated from a genomic DNA library prepared from pools of Onchocerca armillata adults in the plasmid vector pUC12. In dot-blot hybridisations, these two clones do not cross-hybridise significantly with total genomic DNA from O. volvulus, O. gutturosa, O. ochengi, O. gibsoni, O. lienalis, bovine, human, Culicoides nubeculosus, Simulium species or Brugia pahangi, but do hybridise with as little as 100 pg of DNA from two separate geographic isolates of O. armillata. The sequence of pOA1 and pOA5 has been determined and found to contain a repetitive DNA sequence 147 bp in length. These clones can be used as specific and sensitive DNA probes for the identification of O. armillata capable of identifying a single L3 larva.

KW - DNA probe

KW - Onchocerca armillata

KW - Repetitive DNA

KW - Taxonomy

U2 - 10.1016/0166-6851(88)90089-8

DO - 10.1016/0166-6851(88)90089-8

M3 - Article

SN - 0166-6851

VL - 30

SP - 209

EP - 215

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 3

ER -

Cloning and characterization of a species-specific repetitive DNA sequence from Onchocerca armillata

Abstract

Keywords

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