Carriage of potentially pathogenic Escherichia coli in chickens

S. Kariuki; C.F. Gilks; J. Kimari; J. Muyodi; B. Getty; C. Anthony Hart

doi:10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2

Carriage of potentially pathogenic Escherichia coli in chickens

S. Kariuki, C.F. Gilks, J. Kimari, J. Muyodi, B. Getty, C. Anthony Hart

International Public Health

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

DNA-DNA hybridization, cultured cell lines, and transmission electron microscopy were used to study pathogenicity traits of 64 Escherichia coli isolated from apparently healthy chickens from 18 small-scale farms in Thika District, Kenya. A total of 39 (60.9%) isolates hybridized with the eae gene probe for enteropathogenic E. coli (EPEC) whereas another 16 (25%) hybridized with the lt and st gene probes and were categorized as enterotoxigenic E. coli. Electron microscopic examination of the eae probe-positive E coli cultures with the HT-2919A cell line confirmed that they were able to attach intimately and produced effacement typical of EPEC. In addition, negative stain electron microscopy showed that the EPEC strains produced pili that have previously been associated with increased virulence of E. coli infections in chickens. This study has also demonstrated that apparently healthy chickens may carry enteropathogenic E. coli strains.

Original language	English
Pages (from-to)	721-724
Number of pages	4
Journal	Avian Diseases
Volume	46
Issue number	3
DOIs	https://doi.org/10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2
Publication status	Published - 1 Jul 2002

Keywords

Chicken
Enteropathogenic
Escherichia coli

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title = "Carriage of potentially pathogenic Escherichia coli in chickens",

abstract = "DNA-DNA hybridization, cultured cell lines, and transmission electron microscopy were used to study pathogenicity traits of 64 Escherichia coli isolated from apparently healthy chickens from 18 small-scale farms in Thika District, Kenya. A total of 39 (60.9\%) isolates hybridized with the eae gene probe for enteropathogenic E. coli (EPEC) whereas another 16 (25\%) hybridized with the lt and st gene probes and were categorized as enterotoxigenic E. coli. Electron microscopic examination of the eae probe-positive E coli cultures with the HT-2919A cell line confirmed that they were able to attach intimately and produced effacement typical of EPEC. In addition, negative stain electron microscopy showed that the EPEC strains produced pili that have previously been associated with increased virulence of E. coli infections in chickens. This study has also demonstrated that apparently healthy chickens may carry enteropathogenic E. coli strains.",

keywords = "Chicken, Enteropathogenic, Escherichia coli",

author = "S. Kariuki and C.F. Gilks and J. Kimari and J. Muyodi and B. Getty and Hart, \{C. Anthony\}",

year = "2002",

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doi = "10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2",

language = "English",

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TY - JOUR

T1 - Carriage of potentially pathogenic Escherichia coli in chickens

AU - Kariuki, S.

AU - Gilks, C.F.

AU - Kimari, J.

AU - Muyodi, J.

AU - Getty, B.

AU - Hart, C. Anthony

PY - 2002/7/1

Y1 - 2002/7/1

N2 - DNA-DNA hybridization, cultured cell lines, and transmission electron microscopy were used to study pathogenicity traits of 64 Escherichia coli isolated from apparently healthy chickens from 18 small-scale farms in Thika District, Kenya. A total of 39 (60.9%) isolates hybridized with the eae gene probe for enteropathogenic E. coli (EPEC) whereas another 16 (25%) hybridized with the lt and st gene probes and were categorized as enterotoxigenic E. coli. Electron microscopic examination of the eae probe-positive E coli cultures with the HT-2919A cell line confirmed that they were able to attach intimately and produced effacement typical of EPEC. In addition, negative stain electron microscopy showed that the EPEC strains produced pili that have previously been associated with increased virulence of E. coli infections in chickens. This study has also demonstrated that apparently healthy chickens may carry enteropathogenic E. coli strains.

AB - DNA-DNA hybridization, cultured cell lines, and transmission electron microscopy were used to study pathogenicity traits of 64 Escherichia coli isolated from apparently healthy chickens from 18 small-scale farms in Thika District, Kenya. A total of 39 (60.9%) isolates hybridized with the eae gene probe for enteropathogenic E. coli (EPEC) whereas another 16 (25%) hybridized with the lt and st gene probes and were categorized as enterotoxigenic E. coli. Electron microscopic examination of the eae probe-positive E coli cultures with the HT-2919A cell line confirmed that they were able to attach intimately and produced effacement typical of EPEC. In addition, negative stain electron microscopy showed that the EPEC strains produced pili that have previously been associated with increased virulence of E. coli infections in chickens. This study has also demonstrated that apparently healthy chickens may carry enteropathogenic E. coli strains.

KW - Chicken

KW - Enteropathogenic

KW - Escherichia coli

U2 - 10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2

DO - 10.1637/0005-2086(2002)046[0721:coppec]2.0.co;2

M3 - Article

SN - 0005-2086

VL - 46

SP - 721

EP - 724

JO - Avian Diseases

JF - Avian Diseases

IS - 3

ER -

Carriage of potentially pathogenic Escherichia coli in chickens

Abstract

Keywords

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