TY - JOUR
T1 - Binding of Hematin by a New Class of Glutathione Transferase from the Blood-Feeding Parasitic Nematode Haemonchus contortus
AU - Van Rossum, Arjan J.
AU - Jefferies, James R.
AU - Rijsewijk, Frans A.M.
AU - LaCourse, James
AU - Teesdale-Spittle, Paul
AU - Barrett, John
AU - Tait, Andrew
AU - Brophy, Peter M.
PY - 2004/5/1
Y1 - 2004/5/1
N2 - The phase II detoxification system glutathione transferase (GST) is associated with the establishment of parasitic nematode infections within the gastrointestinal environment of the mammalian host. We report the functional analysis of a GST from an important worldwide parasitic nematode of small ruminants, Haemonchus contortus. This GST shows limited activity with a range of classical GST substrates but effectively binds hematin. The high-affinity binding site for hematin was not present in the GST showing the most identity, CE07055 from the free-living nematode Caenorhabditis elegans. This finding suggests that the high-affinity binding of hematin may represent a parasite adaptation to blood or tissue feeding from the host.
AB - The phase II detoxification system glutathione transferase (GST) is associated with the establishment of parasitic nematode infections within the gastrointestinal environment of the mammalian host. We report the functional analysis of a GST from an important worldwide parasitic nematode of small ruminants, Haemonchus contortus. This GST shows limited activity with a range of classical GST substrates but effectively binds hematin. The high-affinity binding site for hematin was not present in the GST showing the most identity, CE07055 from the free-living nematode Caenorhabditis elegans. This finding suggests that the high-affinity binding of hematin may represent a parasite adaptation to blood or tissue feeding from the host.
U2 - 10.1128/iai.72.5.2780-2790.2004
DO - 10.1128/iai.72.5.2780-2790.2004
M3 - Article
SN - 0019-9567
VL - 72
SP - 2780
EP - 2790
JO - Infection and Immunity
JF - Infection and Immunity
IS - 5
ER -