Antioxidant gene expression in the blood-feeding fly Glossina morsitans morsitans

R. J. L. Munks; Mauricio R.V. Sant'Anna; W. Grail; W. Gibson; T. Igglesden; M. Yoshiyama; Stella Lehane; Mike Lehane

doi:10.1111/j.1365-2583.2005.00579.x

Antioxidant gene expression in the blood-feeding fly Glossina morsitans morsitans

R. J. L. Munks, Mauricio R.V. Sant'Anna, W. Grail, W. Gibson, T. Igglesden, M. Yoshiyama, Stella Lehane, Mike Lehane

Vector Biology

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

We report the characterization of 11 antioxidant genes from the tsetse fly Glossina m. morsitans. Through similarity searches which detected homology we suggest that these genes consist of two superoxide dismutases (one with a putative signal peptide), three thioredoxin peroxidases (one with a putative signal peptide), three peroxiredoxins, one further signal peptide-containing peroxidase with its closest similarity to a glutathione peroxidase, one catalase and one thioredoxin reductase. We describe the changes occurring in the expression levels of these genes during fly development, in different adult tissues, in the adult midgut through the digestive cycle and following trypanosome infection. Overall, nine of the 11 genes studied showed responses to changes in physiological circumstance, with the peroxiredoxin group showing the smallest variations throughout.

Original language	English
Pages (from-to)	483-491
Number of pages	9
Journal	Insect Molecular Biology
Volume	14
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2583.2005.00579.x
Publication status	Published - 1 Oct 2005

Keywords

Antioxidant
Glossina
Haem
Immunity
Trypanosome

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10.1111/j.1365-2583.2005.00579.x

Cite this

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title = "Antioxidant gene expression in the blood-feeding fly Glossina morsitans morsitans",

abstract = "We report the characterization of 11 antioxidant genes from the tsetse fly Glossina m. morsitans. Through similarity searches which detected homology we suggest that these genes consist of two superoxide dismutases (one with a putative signal peptide), three thioredoxin peroxidases (one with a putative signal peptide), three peroxiredoxins, one further signal peptide-containing peroxidase with its closest similarity to a glutathione peroxidase, one catalase and one thioredoxin reductase. We describe the changes occurring in the expression levels of these genes during fly development, in different adult tissues, in the adult midgut through the digestive cycle and following trypanosome infection. Overall, nine of the 11 genes studied showed responses to changes in physiological circumstance, with the peroxiredoxin group showing the smallest variations throughout.",

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AU - Munks, R. J. L.

AU - Sant'Anna, Mauricio R.V.

AU - Grail, W.

AU - Gibson, W.

AU - Igglesden, T.

AU - Yoshiyama, M.

AU - Lehane, Stella

AU - Lehane, Mike

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AB - We report the characterization of 11 antioxidant genes from the tsetse fly Glossina m. morsitans. Through similarity searches which detected homology we suggest that these genes consist of two superoxide dismutases (one with a putative signal peptide), three thioredoxin peroxidases (one with a putative signal peptide), three peroxiredoxins, one further signal peptide-containing peroxidase with its closest similarity to a glutathione peroxidase, one catalase and one thioredoxin reductase. We describe the changes occurring in the expression levels of these genes during fly development, in different adult tissues, in the adult midgut through the digestive cycle and following trypanosome infection. Overall, nine of the 11 genes studied showed responses to changes in physiological circumstance, with the peroxiredoxin group showing the smallest variations throughout.

KW - Antioxidant

KW - Glossina

KW - Haem

KW - Immunity

KW - Trypanosome

U2 - 10.1111/j.1365-2583.2005.00579.x

DO - 10.1111/j.1365-2583.2005.00579.x

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EP - 491

JO - Insect Molecular Biology

JF - Insect Molecular Biology

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ER -

Antioxidant gene expression in the blood-feeding fly Glossina morsitans morsitans

Abstract

Keywords

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