An integrated genetic and physical map for the malaria vector Anopheles funestus

Charles Wondji; Richard H. Hunt; Patricia Pignatelli; Keith Steen; Maureen Coetzee; Nora Besansky; Neil Lobo; Frank H. Collins; Janet Hemingway; Hilary Ranson

doi:10.1534/genetics.105.044800

An integrated genetic and physical map for the malaria vector Anopheles funestus

Charles Wondji, Richard H. Hunt, Patricia Pignatelli, Keith Steen, Maureen Coetzee, Nora Besansky, Neil Lobo, Frank H. Collins, Janet Hemingway, Hilary Ranson

Vector Biology

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We have constructed a genetic map of the major African malaria vector, Anopheles funestus, using genetic markers segregating in F-2 progeny from crosses between two strains colonized from different field sites. Genotyping was performed on 174 progeny from three families using 33 microsatellite markers, a single RFLP, and 15 single nucleotide polymorphism (SNP) loci. Four linkage groups were resolved and these were anchored to chromosomes X and 2 and chromosomal arms 3R and 3L by comparison with a physical map of this species. Five markers were linked to the X chromosome, 16 markers to chromosome 2, and 10 and 11 markers to chromosomal arms 3R and 3L, respectively. This significantly increases the number of chromosomally defined genetic markers for this species and will facilitate the identification of genes controlling epidemiologically important traits such as resistance to insecticides or vector competence.

Original language	English
Pages (from-to)	1779-1787
Number of pages	9
Journal	Genetics
Volume	171
Issue number	4
DOIs	https://doi.org/10.1534/genetics.105.044800
Publication status	Published - 1 Dec 2005

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title = "An integrated genetic and physical map for the malaria vector Anopheles funestus",

abstract = "We have constructed a genetic map of the major African malaria vector, Anopheles funestus, using genetic markers segregating in F-2 progeny from crosses between two strains colonized from different field sites. Genotyping was performed on 174 progeny from three families using 33 microsatellite markers, a single RFLP, and 15 single nucleotide polymorphism (SNP) loci. Four linkage groups were resolved and these were anchored to chromosomes X and 2 and chromosomal arms 3R and 3L by comparison with a physical map of this species. Five markers were linked to the X chromosome, 16 markers to chromosome 2, and 10 and 11 markers to chromosomal arms 3R and 3L, respectively. This significantly increases the number of chromosomally defined genetic markers for this species and will facilitate the identification of genes controlling epidemiologically important traits such as resistance to insecticides or vector competence.",

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T1 - An integrated genetic and physical map for the malaria vector Anopheles funestus

AU - Wondji, Charles

AU - Hunt, Richard H.

AU - Pignatelli, Patricia

AU - Steen, Keith

AU - Coetzee, Maureen

AU - Besansky, Nora

AU - Lobo, Neil

AU - Collins, Frank H.

AU - Hemingway, Janet

AU - Ranson, Hilary

PY - 2005/12/1

Y1 - 2005/12/1

N2 - We have constructed a genetic map of the major African malaria vector, Anopheles funestus, using genetic markers segregating in F-2 progeny from crosses between two strains colonized from different field sites. Genotyping was performed on 174 progeny from three families using 33 microsatellite markers, a single RFLP, and 15 single nucleotide polymorphism (SNP) loci. Four linkage groups were resolved and these were anchored to chromosomes X and 2 and chromosomal arms 3R and 3L by comparison with a physical map of this species. Five markers were linked to the X chromosome, 16 markers to chromosome 2, and 10 and 11 markers to chromosomal arms 3R and 3L, respectively. This significantly increases the number of chromosomally defined genetic markers for this species and will facilitate the identification of genes controlling epidemiologically important traits such as resistance to insecticides or vector competence.

AB - We have constructed a genetic map of the major African malaria vector, Anopheles funestus, using genetic markers segregating in F-2 progeny from crosses between two strains colonized from different field sites. Genotyping was performed on 174 progeny from three families using 33 microsatellite markers, a single RFLP, and 15 single nucleotide polymorphism (SNP) loci. Four linkage groups were resolved and these were anchored to chromosomes X and 2 and chromosomal arms 3R and 3L by comparison with a physical map of this species. Five markers were linked to the X chromosome, 16 markers to chromosome 2, and 10 and 11 markers to chromosomal arms 3R and 3L, respectively. This significantly increases the number of chromosomally defined genetic markers for this species and will facilitate the identification of genes controlling epidemiologically important traits such as resistance to insecticides or vector competence.

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DO - 10.1534/genetics.105.044800

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JF - Genetics

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An integrated genetic and physical map for the malaria vector Anopheles funestus

Abstract

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