An enzyme-linked immunosorbent assay for heat-shock protein 70 in plants

K. G. Adham; C. J. Smith; D. L. Zaidman; Mark Wilkinson

doi:10.1080/09540109109354727

An enzyme-linked immunosorbent assay for heat-shock protein 70 in plants

K. G. Adham, C. J. Smith, D. L. Zaidman, Mark Wilkinson

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6 Citations (Scopus)

Abstract

Procedures are described for the production of antibodies against heat-shock protein 70 (HSP70) from plants and for the development of an enzyme-linked immunosorbent assay (ELISA) for the antigen in crude plant extracts. Competitive and two-site sandwich variants of the ELISA are compared. Results are presented for a preliminary analysis of HSP70 levels in control and heat-shocked mung bean shoots.

Original language	English
Pages (from-to)	29-36
Number of pages	8
Journal	Food and Agricultural Immunology
Volume	3
Issue number	1
DOIs	https://doi.org/10.1080/09540109109354727
Publication status	Published - 1 Jan 1991
Externally published	Yes

Keywords

enzyme-linked immunosorbent assay
heat-shock protein
HSP70

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10.1080/09540109109354727

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title = "An enzyme-linked immunosorbent assay for heat-shock protein 70 in plants",

abstract = "Procedures are described for the production of antibodies against heat-shock protein 70 (HSP70) from plants and for the development of an enzyme-linked immunosorbent assay (ELISA) for the antigen in crude plant extracts. Competitive and two-site sandwich variants of the ELISA are compared. Results are presented for a preliminary analysis of HSP70 levels in control and heat-shocked mung bean shoots.",

keywords = "enzyme-linked immunosorbent assay, heat-shock protein, HSP70",

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year = "1991",

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T1 - An enzyme-linked immunosorbent assay for heat-shock protein 70 in plants

AU - Adham, K. G.

AU - Smith, C. J.

AU - Zaidman, D. L.

AU - Wilkinson, Mark

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Procedures are described for the production of antibodies against heat-shock protein 70 (HSP70) from plants and for the development of an enzyme-linked immunosorbent assay (ELISA) for the antigen in crude plant extracts. Competitive and two-site sandwich variants of the ELISA are compared. Results are presented for a preliminary analysis of HSP70 levels in control and heat-shocked mung bean shoots.

AB - Procedures are described for the production of antibodies against heat-shock protein 70 (HSP70) from plants and for the development of an enzyme-linked immunosorbent assay (ELISA) for the antigen in crude plant extracts. Competitive and two-site sandwich variants of the ELISA are compared. Results are presented for a preliminary analysis of HSP70 levels in control and heat-shocked mung bean shoots.

KW - enzyme-linked immunosorbent assay

KW - heat-shock protein

KW - HSP70

U2 - 10.1080/09540109109354727

DO - 10.1080/09540109109354727

M3 - Article

SN - 0954-0105

VL - 3

SP - 29

EP - 36

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

IS - 1

ER -

An enzyme-linked immunosorbent assay for heat-shock protein 70 in plants

Abstract

Keywords

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