A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers.

Bilali Kabula; Patrick Tungu; Emily Rippon; Keith Steen; William Kisinza; Stephen Magesa; Franklin Mosha; Martin Donnelly

doi:10.1186/s12936-016-1331-5

A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers.

Bilali Kabula, Patrick Tungu, Emily Rippon, Keith Steen, William Kisinza, Stephen Magesa, Franklin Mosha, Martin Donnelly

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

BACKGROUND

The success of malaria vector control is threatened by widespread pyrethroid insecticide resistance. However, the extent to which insecticide resistance impacts transmission is unclear. The objective of this study was to examine the association between the DDT/pyrethroid knockdown resistance mutation Vgsc-1014S, commonly termed kdr, and infection with Plasmodium falciparum sporozoites in Anopheles gambiae.

METHODS

WHO standard methods were used to characterize susceptibility of wild female mosquitoes to 0.05 % deltamethrin. PCR-based molecular diagnostics were used to identify mosquitoes to species and to genotype at the Vgsc-L1014S locus. ELISAs were used to detect the presence of P. falciparum sporozoites and for blood meal identification.

RESULTS

Anopheles mosquitoes were resistant to deltamethrin with mortality rates of 77.7 % [95 % CI 74.9-80.3 %]. Of 545 mosquitoes genotyped 96.5 % were A. gambiae s.s. and 3.5 % were Anopheles arabiensis. The Vgsc-1014S mutation was detected in both species. Both species were predominantly anthropophagic. In A. gambiae s.s., Vgsc-L1014S genotype was significantly associated with deltamethrin resistance (χ2 = 11.2; p < 0.001). The P. falciparum sporozoite infection rate was 4.2 %. There was a significant association between the presence of sporozoites and Vgsc-L1014S genotype in A. gambiae s.s. (χ2 = 4.94; p = 0.026).

CONCLUSIONS

One marker, Vgsc-1014S, was associated with insecticide resistance and P. falciparum infection in wild-caught mixed aged populations of A. gambiae s.s. thereby showing how resistance may directly impact transmission.

Original language	English
Article number	289
Pages (from-to)	289
Journal	Malaria Journal
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12936-016-1331-5
Publication status	Published - 23 May 2016

Keywords

Anopheles gambiae
Kdr
L1014S
Plasmodium falciparum
Tanzania
Vgsc

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Kabula, B., Tungu, P., Rippon, E., Steen, K., Kisinza, W., Magesa, S., Mosha, F., & Donnelly, M. (2016). A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers. Malaria Journal, 15(1), 289. Article 289. https://doi.org/10.1186/s12936-016-1331-5

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title = "A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers.",

abstract = "BACKGROUNDThe success of malaria vector control is threatened by widespread pyrethroid insecticide resistance. However, the extent to which insecticide resistance impacts transmission is unclear. The objective of this study was to examine the association between the DDT/pyrethroid knockdown resistance mutation Vgsc-1014S, commonly termed kdr, and infection with Plasmodium falciparum sporozoites in Anopheles gambiae.METHODSWHO standard methods were used to characterize susceptibility of wild female mosquitoes to 0.05 % deltamethrin. PCR-based molecular diagnostics were used to identify mosquitoes to species and to genotype at the Vgsc-L1014S locus. ELISAs were used to detect the presence of P. falciparum sporozoites and for blood meal identification.RESULTSAnopheles mosquitoes were resistant to deltamethrin with mortality rates of 77.7 % [95 % CI 74.9-80.3 %]. Of 545 mosquitoes genotyped 96.5 % were A. gambiae s.s. and 3.5 % were Anopheles arabiensis. The Vgsc-1014S mutation was detected in both species. Both species were predominantly anthropophagic. In A. gambiae s.s., Vgsc-L1014S genotype was significantly associated with deltamethrin resistance (χ2 = 11.2; p < 0.001). The P. falciparum sporozoite infection rate was 4.2 %. There was a significant association between the presence of sporozoites and Vgsc-L1014S genotype in A. gambiae s.s. (χ2 = 4.94; p = 0.026).CONCLUSIONSOne marker, Vgsc-1014S, was associated with insecticide resistance and P. falciparum infection in wild-caught mixed aged populations of A. gambiae s.s. thereby showing how resistance may directly impact transmission.",

keywords = "Anopheles gambiae, Kdr, L1014S, Plasmodium falciparum, Tanzania, Vgsc",

author = "Bilali Kabula and Patrick Tungu and Emily Rippon and Keith Steen and William Kisinza and Stephen Magesa and Franklin Mosha and Martin Donnelly",

year = "2016",

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Kabula, B, Tungu, P, Rippon, E, Steen, K, Kisinza, W, Magesa, S, Mosha, F & Donnelly, M 2016, 'A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers.', Malaria Journal, vol. 15, no. 1, 289, pp. 289. https://doi.org/10.1186/s12936-016-1331-5

A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers. / Kabula, Bilali; Tungu, Patrick; Rippon, Emily et al.
In: Malaria Journal, Vol. 15, No. 1, 289, 23.05.2016, p. 289.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A significant association between deltamethrin resistance, Plasmodium falciparum infection and the Vgsc-1014S resistance mutation in Anopheles gambiae highlights the epidemiological importance of resistance markers.

AU - Kabula, Bilali

AU - Tungu, Patrick

AU - Rippon, Emily

AU - Steen, Keith

AU - Kisinza, William

AU - Magesa, Stephen

AU - Mosha, Franklin

AU - Donnelly, Martin

PY - 2016/5/23

Y1 - 2016/5/23

N2 - BACKGROUNDThe success of malaria vector control is threatened by widespread pyrethroid insecticide resistance. However, the extent to which insecticide resistance impacts transmission is unclear. The objective of this study was to examine the association between the DDT/pyrethroid knockdown resistance mutation Vgsc-1014S, commonly termed kdr, and infection with Plasmodium falciparum sporozoites in Anopheles gambiae.METHODSWHO standard methods were used to characterize susceptibility of wild female mosquitoes to 0.05 % deltamethrin. PCR-based molecular diagnostics were used to identify mosquitoes to species and to genotype at the Vgsc-L1014S locus. ELISAs were used to detect the presence of P. falciparum sporozoites and for blood meal identification.RESULTSAnopheles mosquitoes were resistant to deltamethrin with mortality rates of 77.7 % [95 % CI 74.9-80.3 %]. Of 545 mosquitoes genotyped 96.5 % were A. gambiae s.s. and 3.5 % were Anopheles arabiensis. The Vgsc-1014S mutation was detected in both species. Both species were predominantly anthropophagic. In A. gambiae s.s., Vgsc-L1014S genotype was significantly associated with deltamethrin resistance (χ2 = 11.2; p < 0.001). The P. falciparum sporozoite infection rate was 4.2 %. There was a significant association between the presence of sporozoites and Vgsc-L1014S genotype in A. gambiae s.s. (χ2 = 4.94; p = 0.026).CONCLUSIONSOne marker, Vgsc-1014S, was associated with insecticide resistance and P. falciparum infection in wild-caught mixed aged populations of A. gambiae s.s. thereby showing how resistance may directly impact transmission.

AB - BACKGROUNDThe success of malaria vector control is threatened by widespread pyrethroid insecticide resistance. However, the extent to which insecticide resistance impacts transmission is unclear. The objective of this study was to examine the association between the DDT/pyrethroid knockdown resistance mutation Vgsc-1014S, commonly termed kdr, and infection with Plasmodium falciparum sporozoites in Anopheles gambiae.METHODSWHO standard methods were used to characterize susceptibility of wild female mosquitoes to 0.05 % deltamethrin. PCR-based molecular diagnostics were used to identify mosquitoes to species and to genotype at the Vgsc-L1014S locus. ELISAs were used to detect the presence of P. falciparum sporozoites and for blood meal identification.RESULTSAnopheles mosquitoes were resistant to deltamethrin with mortality rates of 77.7 % [95 % CI 74.9-80.3 %]. Of 545 mosquitoes genotyped 96.5 % were A. gambiae s.s. and 3.5 % were Anopheles arabiensis. The Vgsc-1014S mutation was detected in both species. Both species were predominantly anthropophagic. In A. gambiae s.s., Vgsc-L1014S genotype was significantly associated with deltamethrin resistance (χ2 = 11.2; p < 0.001). The P. falciparum sporozoite infection rate was 4.2 %. There was a significant association between the presence of sporozoites and Vgsc-L1014S genotype in A. gambiae s.s. (χ2 = 4.94; p = 0.026).CONCLUSIONSOne marker, Vgsc-1014S, was associated with insecticide resistance and P. falciparum infection in wild-caught mixed aged populations of A. gambiae s.s. thereby showing how resistance may directly impact transmission.

KW - Anopheles gambiae

KW - Kdr

KW - L1014S

KW - Plasmodium falciparum

KW - Tanzania

KW - Vgsc

U2 - 10.1186/s12936-016-1331-5

DO - 10.1186/s12936-016-1331-5

M3 - Article

SN - 1475-2875

VL - 15

SP - 289

JO - Malaria Journal

JF - Malaria Journal

IS - 1

M1 - 289

ER -