A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells

Youqiang Ke; Mark Wilkinson; David G. Fernig; John A. Smith; Philip S. Rudland; Roger Barraclough

doi:10.1016/0167-4781(92)90029-y

A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells

Youqiang Ke, Mark Wilkinson, David G. Fernig, John A. Smith, Philip S. Rudland, Roger Barraclough

University of Liverpool

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.

Original language	English
Pages (from-to)	307-310
Number of pages	4
Journal	Biochimica Et Biophysica Acta-Gene Structure and Expression
Volume	1131
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(92)90029-y
Publication status	Published - 15 Jul 1992
Externally published	Yes

Keywords

(Human)
Basic fibroblast growth factor
Recombinant protein

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10.1016/0167-4781(92)90029-y

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title = "A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells",

abstract = "Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.",

keywords = "(Human), Basic fibroblast growth factor, Recombinant protein",

author = "Youqiang Ke and Mark Wilkinson and Fernig, \{David G.\} and Smith, \{John A.\} and Rudland, \{Philip S.\} and Roger Barraclough",

year = "1992",

month = jul,

day = "15",

doi = "10.1016/0167-4781(92)90029-y",

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journal = "Biochimica Et Biophysica Acta-Gene Structure and Expression",

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AU - Ke, Youqiang

AU - Wilkinson, Mark

AU - Fernig, David G.

AU - Smith, John A.

AU - Rudland, Philip S.

AU - Barraclough, Roger

PY - 1992/7/15

Y1 - 1992/7/15

N2 - Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.

AB - Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.

KW - (Human)

KW - Basic fibroblast growth factor

KW - Recombinant protein

U2 - 10.1016/0167-4781(92)90029-y

DO - 10.1016/0167-4781(92)90029-y

M3 - Article

VL - 1131

SP - 307

EP - 310

JO - Biochimica Et Biophysica Acta-Gene Structure and Expression

JF - Biochimica Et Biophysica Acta-Gene Structure and Expression

IS - 3

ER -

A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells

Abstract

Keywords

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