A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin

Ruben C. Hartkoorn; Saye Khoo; David J. Back; John F. Tjia; Catriona J. Waitt; Masautso Chaponda; Geraint Davies; Alison Ardrey; Samantha Ashleigh; Steve Ward

doi:10.1016/j.jchromb.2007.07.005

A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin

Ruben C. Hartkoorn, Saye Khoo, David J. Back, John F. Tjia, Catriona J. Waitt, Masautso Chaponda, Geraint Davies, Alison Ardrey, Samantha Ashleigh, Steve Ward

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Rifampicin is active against both intracellular and extracellular Mycobacterium tuberculosis. The ability to measure rifampicin drug concentrations in both plasma and in cells may be useful in evaluating the suitability of dosage regimens for populations and individuals. Here a novel simple. precise and accurate method for the quantification of rifampicin in both cells and plasma is reported. Sample proteins were precipitated with acetonitrile containing the internal standard and then diluted with water. Aliquots of supernatant were then injected into the HPLC-MS system for chromatographic separation and detection. Rifampicin calibration curves encompassed concentrations from 100 to 12,800 ng/mL. Intraand inter-assay precision and accuracy were determined using low, medium and high concentration quality control samples and was found to be within 10% in all cases. Rifampicin concentrations were found to be unaffected by freeze-thaw cycles, but were significantly affected by heat-inactivation (58 degrees C 40 min). This assay was successfully utilised to determine the pharmacokinetic profile of rifampicin in plasma and peripheral blood mononuclear cells (PBMC) in 8 tuberculosis patients receiving rifampicin over an 8 h period. (c) 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	76-82
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	857
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2007.07.005
Publication status	Published - 15 Sept 2007

Keywords

Cellular
HPLC-MS
Plasma
Rifampicin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jchromb.2007.07.005

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Hartkoorn, R. C., Khoo, S., Back, D. J., Tjia, J. F., Waitt, C. J., Chaponda, M., Davies, G., Ardrey, A., Ashleigh, S., & Ward, S. (2007). A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 857(1), 76-82. https://doi.org/10.1016/j.jchromb.2007.07.005

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abstract = "Rifampicin is active against both intracellular and extracellular Mycobacterium tuberculosis. The ability to measure rifampicin drug concentrations in both plasma and in cells may be useful in evaluating the suitability of dosage regimens for populations and individuals. Here a novel simple. precise and accurate method for the quantification of rifampicin in both cells and plasma is reported. Sample proteins were precipitated with acetonitrile containing the internal standard and then diluted with water. Aliquots of supernatant were then injected into the HPLC-MS system for chromatographic separation and detection. Rifampicin calibration curves encompassed concentrations from 100 to 12,800 ng/mL. Intraand inter-assay precision and accuracy were determined using low, medium and high concentration quality control samples and was found to be within 10\% in all cases. Rifampicin concentrations were found to be unaffected by freeze-thaw cycles, but were significantly affected by heat-inactivation (58 degrees C 40 min). This assay was successfully utilised to determine the pharmacokinetic profile of rifampicin in plasma and peripheral blood mononuclear cells (PBMC) in 8 tuberculosis patients receiving rifampicin over an 8 h period. (c) 2007 Elsevier B.V. All rights reserved.",

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Hartkoorn, RC, Khoo, S, Back, DJ, Tjia, JF, Waitt, CJ, Chaponda, M, Davies, G, Ardrey, A, Ashleigh, S & Ward, S 2007, 'A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 857, no. 1, pp. 76-82. https://doi.org/10.1016/j.jchromb.2007.07.005

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T1 - A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin

AU - Hartkoorn, Ruben C.

AU - Khoo, Saye

AU - Back, David J.

AU - Tjia, John F.

AU - Waitt, Catriona J.

AU - Chaponda, Masautso

AU - Davies, Geraint

AU - Ardrey, Alison

AU - Ashleigh, Samantha

AU - Ward, Steve

PY - 2007/9/15

Y1 - 2007/9/15

N2 - Rifampicin is active against both intracellular and extracellular Mycobacterium tuberculosis. The ability to measure rifampicin drug concentrations in both plasma and in cells may be useful in evaluating the suitability of dosage regimens for populations and individuals. Here a novel simple. precise and accurate method for the quantification of rifampicin in both cells and plasma is reported. Sample proteins were precipitated with acetonitrile containing the internal standard and then diluted with water. Aliquots of supernatant were then injected into the HPLC-MS system for chromatographic separation and detection. Rifampicin calibration curves encompassed concentrations from 100 to 12,800 ng/mL. Intraand inter-assay precision and accuracy were determined using low, medium and high concentration quality control samples and was found to be within 10% in all cases. Rifampicin concentrations were found to be unaffected by freeze-thaw cycles, but were significantly affected by heat-inactivation (58 degrees C 40 min). This assay was successfully utilised to determine the pharmacokinetic profile of rifampicin in plasma and peripheral blood mononuclear cells (PBMC) in 8 tuberculosis patients receiving rifampicin over an 8 h period. (c) 2007 Elsevier B.V. All rights reserved.

AB - Rifampicin is active against both intracellular and extracellular Mycobacterium tuberculosis. The ability to measure rifampicin drug concentrations in both plasma and in cells may be useful in evaluating the suitability of dosage regimens for populations and individuals. Here a novel simple. precise and accurate method for the quantification of rifampicin in both cells and plasma is reported. Sample proteins were precipitated with acetonitrile containing the internal standard and then diluted with water. Aliquots of supernatant were then injected into the HPLC-MS system for chromatographic separation and detection. Rifampicin calibration curves encompassed concentrations from 100 to 12,800 ng/mL. Intraand inter-assay precision and accuracy were determined using low, medium and high concentration quality control samples and was found to be within 10% in all cases. Rifampicin concentrations were found to be unaffected by freeze-thaw cycles, but were significantly affected by heat-inactivation (58 degrees C 40 min). This assay was successfully utilised to determine the pharmacokinetic profile of rifampicin in plasma and peripheral blood mononuclear cells (PBMC) in 8 tuberculosis patients receiving rifampicin over an 8 h period. (c) 2007 Elsevier B.V. All rights reserved.

KW - Cellular

KW - HPLC-MS

KW - Plasma

KW - Rifampicin

U2 - 10.1016/j.jchromb.2007.07.005

DO - 10.1016/j.jchromb.2007.07.005

M3 - Article

SN - 1570-0232

VL - 857

SP - 76

EP - 82

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1

ER -

A rapid and sensitive HPLC-MS method for the detection of plasma and cellular rifampicin

Abstract

Keywords

UN SDGs

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