A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection

Samantha Donnellan; Shaun Pennington; Alessandra Ruggiero; Carmen Martinez-Rodriguez; Marion Pouget; Jordan Thomas; Steve Ward; Georgios Pollakis; Giancarlo Biagini; William A. Paxton

doi:10.1093/infdis/jiac491

A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection

Samantha Donnellan
, Shaun Pennington
, Alessandra Ruggiero
, Carmen Martinez-Rodriguez
, Marion Pouget
, Jordan Thomas
, Steve Ward
, Georgios Pollakis
, Giancarlo Biagini
, William A. Paxton

Tropical Disease Biology

Liverpool School of Tropical Medicine
Edinburgh Napier University
University of Liverpool
University of Verona
University College Dublin

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.

Original language	English
Pages (from-to)	708-713
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	227
Issue number	5
DOIs	https://doi.org/10.1093/infdis/jiac491
Publication status	Published - 20 Dec 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

coinfection
drug screening
HIV-1
Mycobacterium tuberculosis
tuberculosis

Themes

Maternal, Neonatal, Sexual and Reproductive Health
Tuberculosis and Antimicrobial Resistance

Access to Document

10.1093/infdis/jiac491Licence: CC BY

Jiac491Final published version, 576 KBLicence: CC BY

Cite this

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title = "A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection",

abstract = "M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.",

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T1 - A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection

AU - Donnellan, Samantha

AU - Pennington, Shaun

AU - Ruggiero, Alessandra

AU - Martinez-Rodriguez, Carmen

AU - Pouget, Marion

AU - Thomas, Jordan

AU - Ward, Steve

AU - Pollakis, Georgios

AU - Biagini, Giancarlo

AU - Paxton, William A.

PY - 2022/12/20

Y1 - 2022/12/20

N2 - M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.

AB - M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies.

KW - coinfection

KW - drug screening

KW - HIV-1

KW - Mycobacterium tuberculosis

KW - tuberculosis

U2 - 10.1093/infdis/jiac491

DO - 10.1093/infdis/jiac491

M3 - Article

SN - 0022-1899

VL - 227

SP - 708

EP - 713

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 5

ER -

A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection

Abstract

UN SDGs

Keywords

Themes

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