A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus

Patrick McClure; Rebecca Curran; Steven Boneham; Jonathan Ball

doi:10.1016/s0166-0934(00)00179-8

A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus

Patrick McClure, Rebecca Curran, Steven Boneham, Jonathan Ball

University of Nottingham

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	Journal of Virological Methods
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/s0166-0934(00)00179-8
Publication status	Published - 1 Jul 2000
Externally published	Yes

Keywords

Envelope
gp160
HIV-1
Limiting dilution PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/s0166-0934(00)00179-8

Cite this

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title = "A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus",

abstract = "Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis. (C) 2000 Elsevier Science B.V.",

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T1 - A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus

AU - McClure, Patrick

AU - Curran, Rebecca

AU - Boneham, Steven

AU - Ball, Jonathan

PY - 2000/7/1

Y1 - 2000/7/1

N2 - Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis. (C) 2000 Elsevier Science B.V.

AB - Polymerase chain reaction (PCR) amplification of full-length envelope genes from the human immunodeficiency virus type 1 (HIV-1) directly from uncultured clinical samples is difficult. This paper describes a comparative assessment of the performance of three thermostable polymerases in an HIV-1 full-length envelope gene PCR. The PCR method utilising Expand HiFi polymerase was successful when using DNA samples extracted from a variety of sources including blood, semen and various tissues. This method generated high and specific yields of product from samples containing as little as one copy of HIV-1 proviral DNA. The resulting PCR products were suitable for a variety of downstream analytical methods including DNA sequence analysis. (C) 2000 Elsevier Science B.V.

KW - Envelope

KW - gp160

KW - HIV-1

KW - Limiting dilution PCR

U2 - 10.1016/s0166-0934(00)00179-8

DO - 10.1016/s0166-0934(00)00179-8

M3 - Article

SN - 0166-0934

VL - 88

SP - 73

EP - 80

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus

Abstract

Keywords

UN SDGs

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