A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria

Lovett Lawson; Jian Zhang; Michel K. Gomgnimbou; Saddiq T. Abdurrahman; Stéphanie Le Moullec; Fatima Mohamed; Gertrude N. Uzoewulu; Olumide M. Sogaolu; Khye Seng Goh; Nnamdi Emenyonu; Guislaine Refrégier; Luis Cuevas; Christophe Sola

doi:10.1371/journal.pone.0038409

A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria

Lovett Lawson
, Jian Zhang
, Michel K. Gomgnimbou
, Saddiq T. Abdurrahman
, Stéphanie Le Moullec
, Fatima Mohamed
, Gertrude N. Uzoewulu
, Olumide M. Sogaolu
, Khye Seng Goh
, Nnamdi Emenyonu
, Guislaine Refrégier
, Luis Cuevas
, Christophe Sola

Clinical Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Background

Nigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.

Methodology/Principal Findings

DNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.

Original language	English
Article number	e38409
Pages (from-to)	e38409
Journal	PLoS ONE
Volume	7
Issue number	6
DOIs	https://doi.org/10.1371/journal.pone.0038409
Publication status	Published - 28 Jun 2012

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1371/journal.pone.0038409Licence: CC BY

Plos ONE 7 6 e38409Final published version, 930 KBLicence: CC BY

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Lawson, L., Zhang, J., Gomgnimbou, M. K., Abdurrahman, S. T., Le Moullec, S., Mohamed, F., Uzoewulu, G. N., Sogaolu, O. M., Goh, K. S., Emenyonu, N., Refrégier, G., Cuevas, L., & Sola, C. (2012). A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria. PLoS ONE, 7(6), e38409. Article e38409. https://doi.org/10.1371/journal.pone.0038409

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title = "A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria",

abstract = "BackgroundNigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.Methodology/Principal FindingsDNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38\%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.",

author = "Lovett Lawson and Jian Zhang and Gomgnimbou, \{Michel K.\} and Abdurrahman, \{Saddiq T.\} and \{Le Moullec\}, St{\'e}phanie and Fatima Mohamed and Uzoewulu, \{Gertrude N.\} and Sogaolu, \{Olumide M.\} and Goh, \{Khye Seng\} and Nnamdi Emenyonu and Guislaine Refr{\'e}gier and Luis Cuevas and Christophe Sola",

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Lawson, L, Zhang, J, Gomgnimbou, MK, Abdurrahman, ST, Le Moullec, S, Mohamed, F, Uzoewulu, GN, Sogaolu, OM, Goh, KS, Emenyonu, N, Refrégier, G, Cuevas, L & Sola, C 2012, 'A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria', PLoS ONE, vol. 7, no. 6, e38409, pp. e38409. https://doi.org/10.1371/journal.pone.0038409

TY - JOUR

T1 - A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria

AU - Lawson, Lovett

AU - Zhang, Jian

AU - Gomgnimbou, Michel K.

AU - Abdurrahman, Saddiq T.

AU - Le Moullec, Stéphanie

AU - Mohamed, Fatima

AU - Uzoewulu, Gertrude N.

AU - Sogaolu, Olumide M.

AU - Goh, Khye Seng

AU - Emenyonu, Nnamdi

AU - Refrégier, Guislaine

AU - Cuevas, Luis

AU - Sola, Christophe

PY - 2012/6/28

Y1 - 2012/6/28

N2 - BackgroundNigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.Methodology/Principal FindingsDNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.

AB - BackgroundNigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.Methodology/Principal FindingsDNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.

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DO - 10.1371/journal.pone.0038409

M3 - Article

VL - 7

SP - e38409

JO - PLoS ONE

JF - PLoS ONE

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M1 - e38409

ER -

A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria

Abstract

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