TY - JOUR
T1 - A Molecular Epidemiological and Genetic Diversity Study of Tuberculosis in Ibadan, Nnewi and Abuja, Nigeria
AU - Lawson, Lovett
AU - Zhang, Jian
AU - Gomgnimbou, Michel K.
AU - Abdurrahman, Saddiq T.
AU - Le Moullec, Stéphanie
AU - Mohamed, Fatima
AU - Uzoewulu, Gertrude N.
AU - Sogaolu, Olumide M.
AU - Goh, Khye Seng
AU - Emenyonu, Nnamdi
AU - Refrégier, Guislaine
AU - Cuevas, Luis
AU - Sola, Christophe
PY - 2012/6/28
Y1 - 2012/6/28
N2 - BackgroundNigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.Methodology/Principal FindingsDNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.
AB - BackgroundNigeria has the tenth highest burden of tuberculosis (TB) among the 22 TB high-burden countries in the world. This study describes the biodiversity and epidemiology of drug-susceptible and drug-resistant TB in Ibadan, Nnewi and Abuja, using 409 DNAs extracted from culture positive TB isolates.Methodology/Principal FindingsDNAs extracted from clinical isolates of Mycobacterium tuberculosis complex were studied by spoligotyping and 24 VNTR typing. The Cameroon clade (CAM) was predominant followed by the M. africanum (West African 1) and T (mainly T2) clades. By using a smooth definition of clusters, 32 likely epi-linked clusters related to the Cameroon genotype family and 15 likely epi-linked clusters related to other “modern” genotypes were detected. Eight clusters concerned M. africanum West African 1. The recent transmission rate of TB was 38%. This large study shows that the recent transmission of TB in Nigeria is high, without major regional differences, with MDR-TB clusters. Improvement in the TB control programme is imperative to address the TB control problem in Nigeria.
U2 - 10.1371/journal.pone.0038409
DO - 10.1371/journal.pone.0038409
M3 - Article
VL - 7
SP - e38409
JO - PLoS ONE
JF - PLoS ONE
IS - 6
M1 - e38409
ER -
