A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA

Jonathan Ball; Graham M. Beards; Ulrich Desselberger

doi:10.1016/0166-0934(92)90042-c

A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA

Jonathan Ball, Graham M. Beards, Ulrich Desselberger

University Hospitals Birmingham NHS Foundation Trust

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems.

Original language	English
Pages (from-to)	149-153
Number of pages	5
Journal	Journal of Virological Methods
Volume	37
Issue number	2
DOIs	https://doi.org/10.1016/0166-0934(92)90042-c
Publication status	Published - 1 May 1992
Externally published	Yes

Keywords

Alkaline phosphatase amplification system
ELISA
HIV-1 p24 antigen
Levamisole

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10.1016/0166-0934(92)90042-c

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title = "A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA",

abstract = "Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems.",

keywords = "Alkaline phosphatase amplification system, ELISA, HIV-1 p24 antigen, Levamisole",

author = "Jonathan Ball and Beards, \{Graham M.\} and Ulrich Desselberger",

year = "1992",

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AU - Ball, Jonathan

AU - Beards, Graham M.

AU - Desselberger, Ulrich

PY - 1992/5/1

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N2 - Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems.

AB - Alkaline phosphatase amplification systems increase the sensitivity of enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which levamisole is a component of the substrate buffer. This increases sensitivity by reducing the signal from non-specific alkaline phosphatases. The modified procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was shown to increase the sensitivity 4-fold as compared to the unmodified system. The performance of the modified amplification system is comparable to that of commercially available systems.

KW - Alkaline phosphatase amplification system

KW - ELISA

KW - HIV-1 p24 antigen

KW - Levamisole

U2 - 10.1016/0166-0934(92)90042-c

DO - 10.1016/0166-0934(92)90042-c

M3 - Article

SN - 0166-0934

VL - 37

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EP - 153

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

A modified alkaline phosphatase enzyme amplification system and its application in an HIV antigen ELISA

Abstract

Keywords

UN SDGs

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