A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Francesca Tamarozzi; Helen L. Wright; Huw B. Thomas; Steven W. Edwards; Mark Taylor

doi:10.1016/j.imlet.2014.10.025

A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Francesca Tamarozzi
, Helen L. Wright
, Huw B. Thomas
, Steven W. Edwards
, Mark Taylor

Tropical Disease Biology

Liverpool School of Tropical Medicine
University of Pavia
University of Liverpool

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

Original language	English
Pages (from-to)	194-198
Number of pages	5
Journal	Immunology Letters
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/j.imlet.2014.10.025
Publication status	Published - 28 Oct 2014

Keywords

IL-17A
Interleukin-17
Neutrophil
Wolbachia

Access to Document

10.1016/j.imlet.2014.10.025Licence: CC BY-NC-ND

Cite this

@article{86f090a78a7047b6a926f3db71cbb4f9,

title = "A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry",

abstract = "We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.",

keywords = "IL-17A, Interleukin-17, Neutrophil, Wolbachia",

author = "Francesca Tamarozzi and Wright, \{Helen L.\} and Thomas, \{Huw B.\} and Edwards, \{Steven W.\} and Mark Taylor",

year = "2014",

month = oct,

day = "28",

doi = "10.1016/j.imlet.2014.10.025",

language = "English",

volume = "162",

pages = "194--198",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

AU - Tamarozzi, Francesca

AU - Wright, Helen L.

AU - Thomas, Huw B.

AU - Edwards, Steven W.

AU - Taylor, Mark

PY - 2014/10/28

Y1 - 2014/10/28

N2 - We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

AB - We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

KW - IL-17A

KW - Interleukin-17

KW - Neutrophil

KW - Wolbachia

U2 - 10.1016/j.imlet.2014.10.025

DO - 10.1016/j.imlet.2014.10.025

M3 - Article

SN - 0165-2478

VL - 162

SP - 194

EP - 198

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Abstract

Keywords

Access to Document

Fingerprint

Cite this