A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes

Ted Baughman; Chelsea Peterson; Corrie Ortega; Sarah R Preston; Christopher Paton; Jessica Williams; Amy Guy; Gavin Omodei; Brian Johnson; Helen Williams; Scott L O'Neill; Scott A Ritchie; Stephen L Dobson; Damian Madan

doi:10.1371/journal.pntd.0006142

A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes

Ted Baughman
, Chelsea Peterson
, Corrie Ortega
, Sarah R Preston
, Christopher Paton
, Jessica Williams
, Amy Guy
, Gavin Omodei
, Brian Johnson
, Helen Williams
, Scott L O'Neill
, Scott A Ritchie
, Stephen L Dobson
, Damian Madan

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We investigated alternatives to whole blood for blood feeding of mosquitoes with a focus on improved stability and compatibility with mass rearing programs. In contrast to whole blood, an artificial blood diet of ATP-supplemented plasma was effective in maintaining mosquito populations and was compatible with storage for extended periods refrigerated, frozen, and as a lyophilized powder. The plasma ATP diet supported rearing of both Anopheles and Aedes mosquitoes. It was also effective in rearing Wolbachia-infected Aedes mosquitoes, suggesting compatibility with vector control efforts.

Original language	English
Pages (from-to)	e0006142
Journal	PLoS Neglected Tropical Diseases
Volume	11
Issue number	12
Early online date	29 Dec 2017
DOIs	https://doi.org/10.1371/journal.pntd.0006142
Publication status	E-pub ahead of print - 29 Dec 2017

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10.1371/journal.pntd.0006142Licence: CC BY

A highly stable blood meal alternative forAccepted author manuscript, 2.78 MBLicence: CC BY

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Baughman, T., Peterson, C., Ortega, C., Preston, S. R., Paton, C., Williams, J., Guy, A., Omodei, G., Johnson, B., Williams, H., O'Neill, S. L., Ritchie, S. A., Dobson, S. L., & Madan, D. (2017). A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes. PLoS Neglected Tropical Diseases, 11(12), e0006142. Advance online publication. https://doi.org/10.1371/journal.pntd.0006142

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AU - Paton, Christopher

AU - Williams, Jessica

AU - Guy, Amy

AU - Omodei, Gavin

AU - Johnson, Brian

AU - Williams, Helen

AU - O'Neill, Scott L

AU - Ritchie, Scott A

AU - Dobson, Stephen L

AU - Madan, Damian

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A highly stable blood meal alternative for rearing Aedes and Anopheles mosquitoes

Abstract

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